Preparation of miniantibodies to Azospirillum brasilense Sp245 surface antigens and their use for bacterial detection.
J Immunoassay Immunochem
; 33(2): 115-27, 2012.
Article
em En
| MEDLINE
| ID: mdl-22471602
This article reports the first preparation of miniantibodies to Azospirillum brasilense Sp245 surface antigens by using a combinatorial phage library of sheep antibodies. The prepared phage antibodies were used for the first time for lipopolysaccharide and flagellin detection by dot assay, electro-optical analysis of cell suspensions, and transmission electron microscopy. Interaction of A. brasilense Sp245 with antilipopolysaccharide and antiflagellin phage-displayed miniantibodies caused the magnitude of the electro-optical signal to change considerably. The electro-optical results were in good agreement with the electron microscopic data. This is the first reported possibility of employing phage-displayed miniantibodies in bacterial detection aided by electro-optical analysis of cell suspensions.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Azospirillum brasilense
/
Anticorpos Antibacterianos
/
Antígenos de Bactérias
/
Antígenos de Superfície
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
País/Região como assunto:
America do sul
/
Brasil
Idioma:
En
Revista:
J Immunoassay Immunochem
Assunto da revista:
ALERGIA E IMUNOLOGIA
Ano de publicação:
2012
Tipo de documento:
Article
País de publicação:
Reino Unido