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The contribution of a murine CNS-TB model for the understanding of the host-pathogen interactions in the formation of granulomas.
Zucchi, Fabíola C R; Pelegrini-da-Silva, Adriana; Neder, Luciano; Silva, Célio L; Tsanaclis, Ana Maria C; Takayanagui, Osvaldo M.
Afiliação
  • Zucchi FC; Department of Neuroscience, University of São Paulo at Ribeirão Preto School of Medicine, Ribeirão Preto, SP, Brazil. fabiola.zucchi@uleth.ca
J Neurosci Methods ; 206(1): 88-93, 2012 Apr 30.
Article em En | MEDLINE | ID: mdl-22387263
Central nervous system (CNS) tuberculosis (TB) is the most severe form of TB, characterized morphologically by brain granulomas and tuberculous meningitis (TBM). Experimental strategies for the study of the host-pathogen interaction through the analysis of granulomas and its intrinsic molecular mechanisms could provide new insights into the neuropathology of TB. To verify whether cerebellar mycobacterial infection induces the main features of the disease in human CNS and better understand the physiological mechanisms underlying the disease, we injected bacillus Calmette-Guerin (BCG) into the mouse cerebellum. BCG-induced CNS-TB is characterized by the formation of granulomas and TBM, a build up of bacterial loads in these lesions, and microglial recruitment into the lesion sites. In addition, there is an enhanced expression of signaling molecules such as nuclear factor-κB (NF-κB) and there is a presence of inducible nitric oxide synthase (iNOS) in the lesions and surrounding areas. This murine model of cerebellar CNS-TB was characterized by cellular and biochemical immune responses typically found in the human disease. This model could expand our knowledge about granulomas in TB infection of the cerebellum, and help characterize the physiological mechanisms involved with the progression of this serious illness that is responsible for killing millions people every year.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Tuberculose do Sistema Nervoso Central / Compreensão / Modelos Animais de Doenças / Interações Hospedeiro-Patógeno / Granuloma / Mycobacterium bovis Tipo de estudo: Etiology_studies / Prognostic_studies Limite: Animals Idioma: En Revista: J Neurosci Methods Ano de publicação: 2012 Tipo de documento: Article País de afiliação: Brasil País de publicação: Holanda
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Tuberculose do Sistema Nervoso Central / Compreensão / Modelos Animais de Doenças / Interações Hospedeiro-Patógeno / Granuloma / Mycobacterium bovis Tipo de estudo: Etiology_studies / Prognostic_studies Limite: Animals Idioma: En Revista: J Neurosci Methods Ano de publicação: 2012 Tipo de documento: Article País de afiliação: Brasil País de publicação: Holanda