Your browser doesn't support javascript.
loading
Enzymatic detergent in rat peritoneum. A comparative study with physiological solution.
Arteaga-Torres, Rodolfo; Escartín-Chávez, Minerva; Gutiérrez-Samperio, César; Ruiz-Acosta, Juan Manuel; Alonso-Gallegos, Marco; Lerma-Alvarado, Ricardo.
Afiliação
  • Arteaga-Torres R; Hospital Médica TEC 100, Querétaro, México. rodoldoc@gmail.com
Cir Cir ; 79(3): 230-6, 250-6, 2011.
Article em En, Es | MEDLINE | ID: mdl-22380993
BACKGROUND: Peritoneal washing out with physiological solution with different substances added is useful in peritoneal infections, but the effect of enzymatic detergents, such as quaternary didecyl-dimethyl ammonium compounds (DDAC), used in the sterilization of surgical material is unknown. We undertook this study to determine histological changes (inflammation, fibrosis and new vessel formation) in the peritoneum of Wistar rats after the application of physiological solution or DDAC. METHODS: The minimum inhibitory concentration (MIC) of DDAC for E. coli (512 µg/ml) and E. faecalis (128 µg/ml) was determined. Sixty-three Wistar rats weighing 200 ± 20 g were studied. They were divided into three groups: control: 7 rats were instilled with 3 ml of physiological solution in peritoneal cavity; groups 1 and 2 were instilled with 3 ml of MIC for E. coli and E. faecalis, respectively. These groups were divided into four subgroups of seven animals. In every rat, 1 cm(2) of peritoneum was obtained at 2, 7, 14, and 21 days for histological study with hematoxylin-eosin. Ten fields were evaluated. The data obtained were analyzed with the Mann-Whitney test. RESULTS: There were no significant differences in inflammation, fibrosis and new vessel formation with the physiological solution vs. DDAC at 2, 7, 14, and 21 days (p >0.05), except for inflammation at 2 days in group 2 (p = 0.026), which remitted. CONCLUSIONS: There was no significant difference in changes in rat peritoneum after physiological solution or DDAC application.
Assuntos
Buscar no Google
Adicionar na Minha BVS
Imprimir
XML
PubMed Links

Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Peritônio / Peritonite / Infecções por Bactérias Gram-Positivas / Enterococcus faecalis / Detergentes / Escherichia coli / Infecções por Escherichia coli / Compostos de Amônio Quaternário Tipo de estudo: Etiology_studies Limite: Animals Idioma: En / Es Revista: Cir Cir Ano de publicação: 2011 Tipo de documento: Article País de publicação: México
Buscar no Google
Adicionar na Minha BVS
Imprimir
XML
PubMed Links

Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Peritônio / Peritonite / Infecções por Bactérias Gram-Positivas / Enterococcus faecalis / Detergentes / Escherichia coli / Infecções por Escherichia coli / Compostos de Amônio Quaternário Tipo de estudo: Etiology_studies Limite: Animals Idioma: En / Es Revista: Cir Cir Ano de publicação: 2011 Tipo de documento: Article País de publicação: México