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Differentiation of African components of ancestry to stratify groups in a case-control study of a Brazilian urban population.
Silbiger, Vivian N; Hirata, Mario H; Luchessi, Andre D; Genvigir, Fabiana D V; Cerda, Alvaro; Rodrigues, Alice C; Willrich, Maria A V; Arazi, Simone S; Dorea, Egidio L; Bernik, Marcia M S; Faludi, Andre A; Bertolami, Marcelo C; Santos, Carla; Carracedo, Angel; Salas, Antonio; Freire, Ana; Lareu, Maria Victoria; Phillips, Christopher; Porras-Hurtado, Liliana; Fondevila, Manuel; Hirata, Rosario D C.
Afiliação
  • Silbiger VN; Faculty of Pharmaceutical Sciences, University of Sao Paulo, São Paulo, Brazil. viviansilbiger@usp.br
Genet Test Mol Biomarkers ; 16(6): 524-30, 2012 Jun.
Article em En | MEDLINE | ID: mdl-22288895
BACKGROUND: Balancing the subject composition of case and control groups to create homogenous ancestries between each group is essential for medical association studies. METHODS: We explored the applicability of single-tube 34-plex ancestry informative markers (AIM) single nucleotide polymorphisms (SNPs) to estimate the African Component of Ancestry (ACA) to design a future case-control association study of a Brazilian urban sample. RESULTS: One hundred eighty individuals (107 case group; 73 control group) self-described as white, brown-intermediate or black were selected. The proportions of the relative contribution of a variable number of ancestral population components were similar between case and control groups. Moreover, the case and control groups demonstrated similar distributions for ACA <0.25 and >0.50 categories. Notably a high number of outlier values (23 samples) were observed among individuals with ACA <0.25. These individuals presented a high probability of Native American and East Asian ancestral components; however, no individuals originally giving these self-described ancestries were observed in this study. CONCLUSIONS: The strategy proposed for the assessment of ancestry and adjustment of case and control groups for an association study is an important step for the proper construction of the study, particularly when subjects are taken from a complex urban population. This can be achieved using a straight forward multiplexed AIM-SNPs assay of highly discriminatory ancestry markers.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: População Urbana / Indígenas Sul-Americanos / Estudos de Casos e Controles / Polimorfismo de Nucleotídeo Único / População Negra / Hipercolesterolemia Tipo de estudo: Observational_studies / Risk_factors_studies Aspecto: Determinantes_sociais_saude Limite: Female / Humans / Male País/Região como assunto: America do sul / Brasil Idioma: En Revista: Genet Test Mol Biomarkers Assunto da revista: BIOLOGIA MOLECULAR / GENETICA Ano de publicação: 2012 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: População Urbana / Indígenas Sul-Americanos / Estudos de Casos e Controles / Polimorfismo de Nucleotídeo Único / População Negra / Hipercolesterolemia Tipo de estudo: Observational_studies / Risk_factors_studies Aspecto: Determinantes_sociais_saude Limite: Female / Humans / Male País/Região como assunto: America do sul / Brasil Idioma: En Revista: Genet Test Mol Biomarkers Assunto da revista: BIOLOGIA MOLECULAR / GENETICA Ano de publicação: 2012 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos