Occurrence of Toxoplasma gondii antibodies in Dasyprocta aguti from Brazil: comparison of diagnostic techniques.
J Zoo Wildl Med
; 42(4): 763-5, 2011 Dec.
Article
em En
| MEDLINE
| ID: mdl-22204080
In this study, the occurrence of antibodies to Toxoplasma gondii in Brazilian agouti (Dasyprocta aguti) was compared by modified agglutination test (MAT) and indirect fluorescent antibody test (IFAT) using anti-capybara conjugate. Sera from 109 animals were tested using MAT (1:25 cut-off) and IFAT (1:16 cut-off); 19% were positive by MAT, and 18% were positive by IFAT. Overall, the 17 IFAT-positive samples were also positive for MAT. The four positive MAT samples with a titer < or = 200 were IFAT negative. All negative samples obtained by MAT matched with the results of the IFAT. Comparing both tests, and considering MAT as the gold standard, the sensitivity of IFAT was 81%, the specificity was 100%, the accuracy was 97%, the positive predictive value (PPV) was 100%, and the negative predictive value 96%. The kappa value agreement was 87.3% (75.1-99.6%). The anti-capybara conjugate can be successfully used to perform IFAT in Brazilian agouti with maximum specificity and PPV.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Doenças dos Roedores
/
Toxoplasma
/
Testes de Aglutinação
/
Anticorpos Antiprotozoários
/
Toxoplasmose Animal
/
Técnica Indireta de Fluorescência para Anticorpo
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
País/Região como assunto:
America do sul
/
Brasil
Idioma:
En
Revista:
J Zoo Wildl Med
Assunto da revista:
MEDICINA VETERINARIA
Ano de publicação:
2011
Tipo de documento:
Article
País de afiliação:
Brasil
País de publicação:
Estados Unidos