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Atorvastatin and hormone therapy effects on APOE mRNA expression in hypercholesterolemic postmenopausal women.
Issa, Mustafa H; Cerda, Alvaro; Genvigir, Fabiana D V; Cavalli, Selma A; Bertolami, Marcelo C; Faludi, Andre A; Hirata, Mario H; Hirata, Rosario D C.
Afiliação
  • Issa MH; Department of Clinical and Toxicological Analysis, School of Pharmaceutical Sciences, University of Sao Paulo, Sao Paulo, SP, Brazil.
J Steroid Biochem Mol Biol ; 128(3-5): 139-44, 2012 Feb.
Article em En | MEDLINE | ID: mdl-22094353
Menopause is associated with changes in lipid levels resulting in increased risk of atherosclerosis and cardiovascular events. Hormone therapy (HT) and atorvastatin have been used to improve lipid profile in postmenopausal women. Effects of HT, atorvastatin and APOE polymorphisms on serum lipids and APOE and LXRA expression were evaluated in 87 hypercholesterolemic postmenopausal women, randomly selected for treatment with atorvastatin (AT, n=17), estrogen or estrogen plus progestagen (HT, n=34) and estrogen or estrogen plus progestagen associated with atorvastatin (HT+AT, n=36). RNA was extracted from peripheral blood mononuclear cells (PBMC) and mRNA expression was measured by TaqMan(®) PCR. APOE ɛ2/ɛ3/ɛ4 genotyping was performed using PCR-RFLP. Total cholesterol (TC), LDL-c and apoB were reduced after each treatment (p<0.001). Triglycerides, VLDL-c and apoAI were reduced only after atorvastatin (p<0.05), whereas triglycerides and VLDL-c were increased after HT (p=0.01). HT women had lower reduction on TC, LDL-c and apoB than AT and HT+AT groups (p<0.05). APOE mRNA expression was reduced after atorvastatin treatment (p=0.03). Although LXRA gene expression was not modified by atorvastatin, it was correlated with APOE mRNA before and after treatments. Basal APOE mRNA expression was not influenced by gene polymorphisms, however the reduction on APOE expression was more pronounced in ɛ3ɛ3 than in ɛ3ɛ4 carriers. Atorvastatin down-regulates APOE mRNA expression and it is modified by APOE genotypes in PBMC from postmenopausal women.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Apolipoproteínas E / Pirróis / Regulação para Baixo / Terapia de Reposição de Estrogênios / Pós-Menopausa / Inibidores de Hidroximetilglutaril-CoA Redutases / Ácidos Heptanoicos / Hipercolesterolemia Tipo de estudo: Clinical_trials Limite: Aged / Female / Humans / Middle aged País/Região como assunto: America do sul / Brasil Idioma: En Revista: J Steroid Biochem Mol Biol Assunto da revista: BIOLOGIA MOLECULAR / BIOQUIMICA Ano de publicação: 2012 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido
Texto completo
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XML
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Apolipoproteínas E / Pirróis / Regulação para Baixo / Terapia de Reposição de Estrogênios / Pós-Menopausa / Inibidores de Hidroximetilglutaril-CoA Redutases / Ácidos Heptanoicos / Hipercolesterolemia Tipo de estudo: Clinical_trials Limite: Aged / Female / Humans / Middle aged País/Região como assunto: America do sul / Brasil Idioma: En Revista: J Steroid Biochem Mol Biol Assunto da revista: BIOLOGIA MOLECULAR / BIOQUIMICA Ano de publicação: 2012 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido