Limited efficiency of universal mini-barcode primers for DNA amplification from desert reptiles, birds and mammals.
Genet Mol Res
; 10(4): 3559-64, 2011 Oct 31.
Article
em En
| MEDLINE
| ID: mdl-22057991
In recent years, DNA barcoding has emerged as a powerful tool for species identification. We report an extended validation of a universal DNA mini-barcode for amplification of 130-bp COI segments from 23 specimens collected from a desert environment, including 11 reptiles, five mammals and seven birds. Besides the standard double-annealing protocol, we also tested a more stringent single-annealing protocol. The PCR success rate for the amplification of the mini-barcode region was: mammals (4/5), reptiles (5/11) and birds (4/7). These findings demonstrate the limited utility of universal primers for mini-barcoding, at least for these vertebrate taxa that we collected from the Saudi Arabian desert.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Répteis
/
Aves
/
DNA
/
Reação em Cadeia da Polimerase
/
Primers do DNA
/
Código de Barras de DNA Taxonômico
/
Mamíferos
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
Genet Mol Res
Assunto da revista:
BIOLOGIA MOLECULAR
/
GENETICA
Ano de publicação:
2011
Tipo de documento:
Article
País de afiliação:
Arábia Saudita
País de publicação:
Brasil