Antimicrobial effect of endodontic solutions used as final irrigants on a dentine biofilm model.
Int Endod J
; 45(2): 162-8, 2012 Feb.
Article
em En
| MEDLINE
| ID: mdl-21985189
AIM: To evaluate the residual biovolume of live bacterial cells, the mean biofilm thickness and the substratum coverage found in mixed biofilms treated with different endodontic irrigant solutions. METHODOLOGY: Twenty-five bovine dentine specimens were infected intraorally using a removable orthodontic device. Five samples were used for each irrigant solution: 2% chlorhexidine, 1% sodium hypochlorite (NaOCl), 10% citric acid, 17% EDTA and distilled water. The solutions were used for 5 min. The samples were stained using the Live/Dead technique and evaluated using a confocal microscope. Differences in the amount of total biovolume (µm(3)), number of surviving cells (µm(3)), mean biofilm thickness (µm) and substratum coverage (%) of the treated biofilms were determined using nonparametric statistical tests (P < 0.05). RESULTS: Similar values of biovolume total, biovolume of live subpopulations and substratum coverage were found in 2% chlorhexidine, 10% citric acid, 17% EDTA and distilled water-treated biofilms (P > 0.05). The lower values of the studied parameters were found in 1% NaOCl-treated dentine (P < 0.05) with the exception of the mean biofilm height criteria that did not reveal significant differences amongst the irrigant solutions (P > 0.05). CONCLUSIONS: One per cent sodium hypochlorite was the only irrigant that had a significant effect on biofilm viability and architecture.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Irrigantes do Canal Radicular
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Biofilmes
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Dentina
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Anti-Infecciosos
Tipo de estudo:
Prognostic_studies
Limite:
Animals
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Humans
Idioma:
En
Revista:
Int Endod J
Ano de publicação:
2012
Tipo de documento:
Article
País de afiliação:
Brasil
País de publicação:
Reino Unido