Mycobiota and mycotoxins in malted barley and brewer's spent grain from Argentinean breweries.
Lett Appl Microbiol
; 53(6): 649-55, 2011 Dec.
Article
em En
| MEDLINE
| ID: mdl-21967240
AIMS: To evaluate mycobiota and aflatoxins B(1) (AFB(1)), B(2) (AFB(2)), G(1) (AFG(1)), G(2) (AFG(2)) and fumonisin B(1) (FB(1)) contamination in different malted barley types and brands and brewer's grain collected from a major Argentinean brewery. METHODS AND RESULTS: Total fungal counts were performed using the plate count method. Aflatoxin B(1), AFB(2), AFG(1), AFG(2) and Zearalenone (ZEA) analyses were performed by thin-layer chromatography (TLC). Fumonisin B(1) was determined by HPLC. Eighty-three percentage of the malted barley (100% M1, 50% M2 and 100% M3) and 61% of brewer's grain samples had a count >1 × 10(4) CFU g(-1). Yeasts were isolated from all malt and brewer's grain samples. Genera containing some of the most important mycotoxin producer species--Fusarium ssp., Aspergillus ssp., Penicillium ssp. and Alternaria ssp.--were isolated from the analysed samples, along with other environmental saprophytic fungi such as Geotrichum ssp., Mucorales and Cladosporium ssp. All samples were contaminated with 104-145 µg kg(-1) FB(1). Eighteen per cent of brewer's grain samples were contaminated with 19-44.52 µg kg(-1) AFB(1). Aflatoxin B(2), AFG(1), AFG(2) and ZEA were not detected in any of the analysed samples. CONCLUSIONS: Fungal and mycotoxin contamination in malt and brewer's grain is an actual risk for animal and human health. SIGNIFICANCE AND IMPACT OF THE STUDY: This study may be useful for assessing the risk of mycotoxins in Argentinean beers and especially in animal feeds.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Hordeum
/
Cerveja
/
Contaminação de Alimentos
/
Micotoxinas
Limite:
Humans
País/Região como assunto:
America do sul
/
Argentina
Idioma:
En
Revista:
Lett Appl Microbiol
Assunto da revista:
MICROBIOLOGIA
Ano de publicação:
2011
Tipo de documento:
Article
País de afiliação:
Argentina
País de publicação:
Reino Unido