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Characterization of Vibrio parahaemolyticus isolated from oysters and mussels in São Paulo, Brazil.
Rojas, Martha Virginia Ribeiro; Matté, Maria Helena; Dropa, Milena; Silva, Miriam Lopes da; Matté, Glavur Rogério.
Afiliação
  • Rojas MV; Public Health Laboratory, School of Public Health, University of São Paulo, SP, Brazil.
Rev Inst Med Trop Sao Paulo ; 53(4): 201-5, 2011.
Article em En | MEDLINE | ID: mdl-21915463
Vibrio parahaemolyticus is a marine bacterium, responsible for gastroenteritis in humans. Most of the clinical isolates produce thermostable direct hemolysin (TDH) and TDH-related hemolysin (TRH) encoded by tdh and trh genes respectively. In this study, twenty-three V. parahaemolyticus, previously isolated from oysters and mussels were analyzed by PCR using specific primers for the 16S rRNA and virulence genes (tdh, trh and tlh) and for resistance to different classes of antibiotics and PFGE. Nineteen isolates were confirmed by PCR as V. parahaemolyticus. The tlh gene was present in 100% of isolates, the tdh gene was identified in two (10.5%) isolates, whereas the gene trh was not detected. Each isolate was resistant to at least one of the nine antimicrobials tested. Additionally, all isolates possessed the blaTEM-116 gene. The presence of this gene in V. parahaemolyticus indicates the possibility of spreading this gene in the environment. Atypical strains of V. parahaemolyticus were also detected in this study.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Ostreidae / Frutos do Mar / Vibrio parahaemolyticus Tipo de estudo: Prognostic_studies Limite: Animals País/Região como assunto: America do sul / Brasil Idioma: En Revista: Rev Inst Med Trop Sao Paulo Ano de publicação: 2011 Tipo de documento: Article País de afiliação: Brasil País de publicação: Brasil

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Ostreidae / Frutos do Mar / Vibrio parahaemolyticus Tipo de estudo: Prognostic_studies Limite: Animals País/Região como assunto: America do sul / Brasil Idioma: En Revista: Rev Inst Med Trop Sao Paulo Ano de publicação: 2011 Tipo de documento: Article País de afiliação: Brasil País de publicação: Brasil