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Molecular characterization and RAPD analysis of Juniperus species from Iran.
Kasaian, J; Behravan, J; Hassany, M; Emami, S A; Shahriari, F; Khayyat, M H.
Afiliação
  • Kasaian J; Biotechnology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran. kasaeeianj1@mums.ac.ir
Genet Mol Res ; 10(2): 1069-74, 2011 Jun 07.
Article em En | MEDLINE | ID: mdl-21710457
The genus Juniperus L. (Cupressaceae), an aromatic evergreen plant, consists of up to 68 species around the world. We classified five species of Juniperus found in Iran using molecular markers to provide a means for molecular identification of Iranian species. Plants were collected (three samples of each species) from two different provinces of Iran (Golestan and East Azarbayejan). The DNA was extracted from the leaves using a Qiagen Dneasy Plant Mini Kit. Amplification was performed using 18 ten-mer RAPD primers. Genetic distances were estimated based on 187 RAPD bands to construct a dendrogram by means of unweighted pair group method of arithmetic means. It was found that J. communis and J. oblonga were differentiated from the other species. Genetic distance values ranged from 0.19 (J. communis and J. oblonga) to 0.68 (J. communis and J. excelsa). Juniperus foetidissima was found to be most similar to J. sabina. Juniperus excelsa subspecies excelsa and J. excelsa subspecies polycarpos formed a distinct group.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Juniperus / Técnica de Amplificação ao Acaso de DNA Polimórfico País/Região como assunto: Asia Idioma: En Revista: Genet Mol Res Assunto da revista: BIOLOGIA MOLECULAR / GENETICA Ano de publicação: 2011 Tipo de documento: Article País de afiliação: Irã País de publicação: Brasil

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Juniperus / Técnica de Amplificação ao Acaso de DNA Polimórfico País/Região como assunto: Asia Idioma: En Revista: Genet Mol Res Assunto da revista: BIOLOGIA MOLECULAR / GENETICA Ano de publicação: 2011 Tipo de documento: Article País de afiliação: Irã País de publicação: Brasil