Studies on induction of delta-aminolevulinic acid synthase, ferrochelatase, cytochrome P-450 and cyclic AMP by phenformin. Chlorpropamide, allylisopropylacetamide and lead in hepatocytes from normal and experimental diabetic rats.
Biochem Pharmacol
; 40(2): 365-72, 1990 Jul 15.
Article
em En
| MEDLINE
| ID: mdl-2165405
The present work demonstrates that phenformin exerted an inducing effect on delta-aminolevulinic acid synthase (ALA-S) and ferrochelatase activities and on cytochrome P-450 content in isolated hepatocytes from rats with experimental diabetes. Similar results were obtained with respect to ALA-S activity and cytochrome P-450 content when chlorpropamide was used. The inducing effect exerted by allylisopropylacetamide (AIA) on ALA-S and ferrochelatase activities in diabetic hepatic cells was markedly greater than that observed in normal hepatocytes. This stimulatory response was not enhanced by adding dibutyryl cyclic AMP (cAMP). When phenformin was added to isolated rat hepatocytes of normal rats, induction of ALA-S and ferrochelatase activities and cytochrome P-450 content was observed only in the presence of added dibutyryl cAMP. Addition of chlorpropamide to this in vitro system did not exert an inducing effect on the same enzymes even in the presence of dibutyryl cAMP. The present results add more experimental evidence about the lability of the heme pathway of diabetic hepatocytes.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fenformin
/
AMP Cíclico
/
Sistema Enzimático do Citocromo P-450
/
Diabetes Mellitus Experimental
/
Ferroquelatase
/
5-Aminolevulinato Sintetase
/
Liases
Limite:
Animals
Idioma:
En
Revista:
Biochem Pharmacol
Ano de publicação:
1990
Tipo de documento:
Article
País de afiliação:
Argentina
País de publicação:
Reino Unido