Influence of the preservation period in silica-gel on the predatory activity of the isolates of Duddingtonia flagrans on infective larvae of cyathostomins (Nematoda: Cyathostominae).
Exp Parasitol
; 128(4): 460-3, 2011 Aug.
Article
em En
| MEDLINE
| ID: mdl-21627962
The continued maintenance of nematophagous fungi predatory activity under laboratory conditions is one of the basic requirements for a successful biological control. The purpose of this study was to evaluate the influence of time on the preservation of the fungus Duddingtonia flagrans (AC001 and CG722) stored in silica-gel for 7 years and their subsequent predatory activity on cyathostomin L(3) larvae in 2% water-agar medium (2% WA). Samples of the isolates AC001 and CG722, originating from vials containing grains of silica-gel sterilized and stored for 7 years, were used. After obtaining fungal conidia, the predation test was conducted over 7 days on the surface of 9.0 cm Petri dishes filled with 2% WA. In the treated groups each Petri dish contained 500 cyathostomin L(3) and conidia of fungal isolates in 2% WA. In the control group (without fungi) the plates contained 500 L(3) in 2% WA. The experimental results showed that isolated AC001 and CG722 were efficient in preying on cyathostomin L(3) (p<0.01) compared to control (without fungus). However, no difference was observed (p>0.01) in the predatory activity of the fungal isolates tested. Comparing the groups, there was a significant reductions of cyathostomin L(3) (p<0.01) of 88.6% and 78.4% on average recovered from the groups treated with the isolates AC001 and CG722, respectively, after 7 days. The results of this test showed that the fungus D. flagrans (AC001 and CG722) stored in silica-gel for at least 7 years maintained its predatory activity on cyathostomin L(3).
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Preservação Biológica
/
Ascomicetos
/
Infecções Equinas por Strongyloidea
/
Strongyloidea
Limite:
Animals
Idioma:
En
Revista:
Exp Parasitol
Ano de publicação:
2011
Tipo de documento:
Article
País de afiliação:
Brasil
País de publicação:
Estados Unidos