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27-hydroxycholesterol induces the transition of MCF7 cells into a mesenchymal phenotype.
Torres, Cristian G; Ramírez, María E; Cruz, Pamela; Epuñan, María J; Valladares, Luis E; Sierralta, Walter D.
Afiliação
  • Torres CG; Laboratorio de Medicina Experimental, Escuela de Medicina Veterinaria, Universidad Andrés Bello, Santiago 8370251, Chile.
Oncol Rep ; 26(2): 389-97, 2011 Aug.
Article em En | MEDLINE | ID: mdl-21567091
A decrease in the expression of E-cadherin and ß-catenin, paralleling the loss of adherens junction complex, was observed in MCF7 cells exposed for longer than 48 h to 2 µM 27-hydroxycholesterol (27OHC), indicating an epithelial-mesenchymal transition (EMT). Upon removal of 27OHC from the culture medium, the cells released by the exposure of 72 h to the oxysterol grew as loosely packed cell groups. In these cells, accumulation of E-cadherin and ß-catenin in the cytoplasm and the prolonged expression of epidermal growth factor receptor 2 (EGFR2/neu) in the plasma membrane were observed, suggesting that the acquired phenotype was related to the expression of this tyrosine kinase-growth factor receptor. The results presented here are discussed on the basis of the claimed relationship between 27OHC, hypercholesterolemia, macrophage infiltration and therapy-resistant ERα+ breast cancer incidence.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Neoplasias da Mama / Transição Epitelial-Mesenquimal / Hidroxicolesteróis Limite: Animals / Female / Humans Idioma: En Revista: Oncol Rep Assunto da revista: NEOPLASIAS Ano de publicação: 2011 Tipo de documento: Article País de afiliação: Chile País de publicação: Grécia

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Neoplasias da Mama / Transição Epitelial-Mesenquimal / Hidroxicolesteróis Limite: Animals / Female / Humans Idioma: En Revista: Oncol Rep Assunto da revista: NEOPLASIAS Ano de publicação: 2011 Tipo de documento: Article País de afiliação: Chile País de publicação: Grécia