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Reduction of the amount of intestinal secretory IgA in fulminant hepatic failure.
Dong-Yan, Liu; Weiguo, Jiang; Pei, Liu.
Afiliação
  • Dong-Yan L; Research Center, China Medical University Affiliated Shengjing Hospital and Key Laboratory of Congenital Malformation Research, Ministry of Health, Shenyang, China.
Braz J Med Biol Res ; 44(5): 477-82, 2011 May.
Article em En | MEDLINE | ID: mdl-21519636
Intestinal barrier dysfunction plays an important role in spontaneous bacterial peritonitis. In the present study, changes in the intestinal barrier with regard to levels of secretory immunoglobulin A (SIgA) and its components were studied in fulminant hepatic failure (FHF). Immunohistochemistry and double immunofluorescent staining were used to detect intestinal IgA, the secretory component (SC) and SIgA in patients with FHF (20 patients) and in an animal model with FHF (120 mice). Real-time PCR was used to detect intestinal SC mRNA in the animal model with FHF. Intestinal SIgA, IgA, and SC staining in patients with FHF was significantly weaker than in the normal control group (30 patients). Intestinal IgA and SC staining was significantly weaker in the animal model with FHF than in the control groups (normal saline: 30 mice; lipopolysaccharide: 50 mice; D-galactosamine: 50 mice; FHF: 120 mice). SC mRNA of the animal model with FHF at 2, 6, and 9 h after injection was 0.4 ± 0.02, 0.3 ± 0.01, 0.09 ± 0.01, respectively. SC mRNA of the animal model with FHF was significantly decreased compared to the normal saline group (1.0 ± 0.02) and lipopolysaccharide group (0.89 ± 0.01). The decrease in intestinal SIgA and SC induced failure of the intestinal immunologic barrier and the attenuation of gut immunity in the presence of FHF.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Imunoglobulina A Secretora / Falência Hepática Aguda Tipo de estudo: Observational_studies Limite: Animals / Humans Idioma: En Revista: Braz J Med Biol Res Ano de publicação: 2011 Tipo de documento: Article País de afiliação: China País de publicação: Brasil

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Imunoglobulina A Secretora / Falência Hepática Aguda Tipo de estudo: Observational_studies Limite: Animals / Humans Idioma: En Revista: Braz J Med Biol Res Ano de publicação: 2011 Tipo de documento: Article País de afiliação: China País de publicação: Brasil