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Evaluation of the effects of Quercetin and Kaempherol on the surface of MT-2 cells visualized by atomic force microscopy.
Coelho-Dos-Reis, Jordana Grazziela A; Gomes, Orlando Ab; Bortolini, Dener E; Martins, Marina L; Almeida, Marcia R; Martins, Camila S; Carvalho, Luciana D; Souza, Jaqueline G; Vilela, Jose Mario C; Andrade, Margareth S; Barbosa-Stancioli, Edel Figueiredo.
Afiliação
  • Coelho-Dos-Reis JG; Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais - UFMG, Belo Horizonte, Brazil.
J Virol Methods ; 174(1-2): 47-52, 2011 Jun.
Article em En | MEDLINE | ID: mdl-21507333
This study investigated the anti-viral effects of the polyphenolic compounds Quercetin and Kaempherol on the release of HTLV-1 from the surface of MT-2 cells. Atomic force microscopy (AFM) was used to scan the surface of the MT-2 cells. MT-2 cells were fixed with 100% methanol on round glass lamina or cleaved mica and dried under UV light and laminar flow. The images were captured on a Multimode equipment monitored by a NanoScope IIId controller from Veeco Instruments Inc operated in tapping mode and equipped with phase-imaging hardware. The images demonstrated viral budding structures 131 ± 57 nm in size, indicating profuse viral budding. Interestingly, cell-free viruses and budding structures visualized on the surface of cells were less common when MT-2 was incubated with Quercetin, and no particles were seen on the surface of cells incubated with Kaempherol. In summary, these data indicate that HTLV-1 is budding constantly from the MT-2 cell surface and that polyphenolic compounds were able to reduce this viral release. Biological samples were analyzed with crude cell preparations just after cultivation in the presence of Quercetin and Kaempherol, showing that the AFM technique is a rapid and powerful tool for analysis of antiviral activity of new biological compounds.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Antivirais / Quercetina / Linfócitos T / Vírus Linfotrópico T Tipo 1 Humano / Quempferóis / Liberação de Vírus Idioma: En Revista: J Virol Methods Ano de publicação: 2011 Tipo de documento: Article País de afiliação: Brasil País de publicação: Holanda

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Antivirais / Quercetina / Linfócitos T / Vírus Linfotrópico T Tipo 1 Humano / Quempferóis / Liberação de Vírus Idioma: En Revista: J Virol Methods Ano de publicação: 2011 Tipo de documento: Article País de afiliação: Brasil País de publicação: Holanda