[Massive production of Entamoeba histolytica cysts under axenic conditions]. / Producción masiva de quistes de Entamoeba histolytica en condiciones axénicas.
Gac Med Mex
; 126(4): 307-13, 1990.
Article
em Es
| MEDLINE
| ID: mdl-2093567
After an exponential phase of growth, HK9 strain amebas, kept in the axenic medium PEHPS, spontaneously acquire a form morphologically similar to various natural cysts, as well as a resistance to hypotonic shock, due to the effect of a wall, partially composed of polysaccharides. The number of differentiated amebas increases gradually, although their viability diminishes, in function of the incubation time. On the ninth day, 96% of the population is made up of these cells, although only 6% are viable. The ultramicroscopic structure of the great majority of differentiated amebas corresponds to that of immature cysts. These, and the PEHPS medium, constitute a good model for a characterization of the start of the differentiation of E. histolytica, and open the opportunity of obtaining in axenic conditions, massive cultures of mature cysts.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Entamoeba histolytica
/
Vida Livre de Germes
Limite:
Animals
Idioma:
Es
Revista:
Gac Med Mex
Ano de publicação:
1990
Tipo de documento:
Article
País de publicação:
México