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Evaluation of Lionex TB kits and mycobacterial antigens for IgG and IgA detection in cerebrospinal fluid from tuberculosis meningitis patients.
Sardella, Isabela Gama; Singh, Mahavir; Kumpfer, Susanne; Heringer, Rafael Ribeiro; Saad, Maria Helena Féres; Sohler, Marzia Puccioni.
Afiliação
  • Sardella IG; Laboratório de Microbiologia Celular, Instituto Oswaldo Cruz-Fiocruz, Av Brasil 4365, 21045-900 Rio de Janeiro, RJ, Brasil.
Mem Inst Oswaldo Cruz ; 105(5): 722-8, 2010 Aug.
Article em En | MEDLINE | ID: mdl-20835625
To evaluate commercial Lionex TB together with four antigens of Mycobacterium tuberculosis (MPT-64, MT10.3, 16 kDa and 38 kDa) for IgG and IgA cerebrospinal fluid (CSF) detection in the diagnosis of tuberculosis meningitis (TBM) with CSF negative acid-fast bacilli staining, 19 cases of TBM, 64 cases of other infectious meningoencephalitis and 73 cases of other neurological disorders were tested by enzyme linked immunosorbent assay. IgA-MPT-64 and IgG Lionex showed the highest sensitivities, specificities, positive predictive value and negative predictive value (63.2%, 47.4%; 95%, 93.7%; 40%, 98% and 28.4%, 97.1%, respectively). However, while grey zone was 12.7% and 6%, respectively, lowering sensitivity but maintains high specificity (>or= 95%). High protein concentration in CSF was associated with antibody positivity CSF/HIV+ which did not influence the sensitivity of both tests. To our knowledge, this is the first description of IgA-MPT-64 and IgG Lionex antibodies in CSF-TBM and, although there is good specificity, adjustments are needed based on antigen composition to enhance sensitivity.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Tuberculose Meníngea / Imunoglobulina A / Imunoglobulina G / Anticorpos Antibacterianos / Mycobacterium tuberculosis / Antígenos de Bactérias Tipo de estudo: Diagnostic_studies / Evaluation_studies / Prognostic_studies Limite: Humans Idioma: En Revista: Mem Inst Oswaldo Cruz Ano de publicação: 2010 Tipo de documento: Article País de afiliação: Brasil País de publicação: Brasil

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Tuberculose Meníngea / Imunoglobulina A / Imunoglobulina G / Anticorpos Antibacterianos / Mycobacterium tuberculosis / Antígenos de Bactérias Tipo de estudo: Diagnostic_studies / Evaluation_studies / Prognostic_studies Limite: Humans Idioma: En Revista: Mem Inst Oswaldo Cruz Ano de publicação: 2010 Tipo de documento: Article País de afiliação: Brasil País de publicação: Brasil