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Strategies to improve pregnancy per insemination using sex-sorted semen in dairy heifers detected in estrus.
Sá Filho, M F; Ayres, H; Ferreira, R M; Nichi, M; Fosado, M; Campos Filho, E P; Baruselli, P S.
Afiliação
  • Sá Filho MF; Departamento de Reprodução Animal, FMVZ-USP, CEP 05.508-000, São Paulo, SP, Brasil.
Theriogenology ; 74(9): 1636-42, 2010 Dec.
Article em En | MEDLINE | ID: mdl-20833421
The objective was to improve pregnancy per artificial insemination (P/AI; 35-42 d after AI) in virgin Jersey heifers bred by AI of sex-sorted semen after being detected in estrus. Giving 100 µg of GnRH at first detection of estrus, with AI 12 h later, did not affect P/AI in Experiment I [GnRH = 47.2% (100/212) vs. No GnRH = 51.7% (104/201); P = 0.38] or Experiment II [GnRH = 53.1% (137/258) vs. No GnRH = 48.6% (122/251); P = 0.43]. In these two experiments, estrus detection was done with tail-head chalk or a HeatWatch(®) system, respectively. In Experiment III, a single insemination dose (2.1 × 106 sperm) 12 h after estrus detection (n = 193), a double dose at 12 h (n = 193), or a double dose involving insemination 12 and 24 h after estrus detection (n = 190) did not affect P/AI (87/193 = 45.1%, 85/193 = 44.0%, and 94/190 = 49.5%, respectively; P = 0.51). However, P/AI was influenced by the number of AI service (First, 115/208 = 55.3%(a); Second, 94/204 = 46.1%(a); and Third, 57/165 = 34.8%(b); P = 0.004). In Experiment IV, the P/AI of heifers inseminated from 12 to 16 h after the onset of estrus (40/106 = 37.7%) was less (P = 0.03) than those inseminated from 16.1 to 20 h (85/164 = 51.8%), and 20.1 to 24 h (130/234 = 55.6%). However, the P/AI for heifers inseminated from 24.1 to 30 h (61/134 = 45.5%) did not differ from that of any other interval. In conclusion, in Jersey heifers inseminated with sex-sorted semen, P/AI was not significantly affected by giving GnRH at detection of estrus or a double insemination dose, but it was higher with AI 16.1 to 24 h vs. 12 to 16 h after the onset of estrus.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Sêmen / Bovinos / Inseminação Artificial Limite: Animals / Pregnancy Idioma: En Revista: Theriogenology Ano de publicação: 2010 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Sêmen / Bovinos / Inseminação Artificial Limite: Animals / Pregnancy Idioma: En Revista: Theriogenology Ano de publicação: 2010 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos