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Genetic variability among Anopheles species belonging to the Nyssorhynchus and Anopheles subgenera in the Amazon region.
Moroni, Raquel Borges; Maia, Juracy de Freitas; Tadei, Wanderli Pedro; Santos, Joselita Maria Mendes dos.
Afiliação
  • Moroni RB; Department of Parasitology, Institute of Biological Sciences, Federal University of Amazon, Manaus, AM, Brazil. borgesraquel@hotmail.com
Rev Soc Bras Med Trop ; 43(4): 409-15, 2010.
Article em En | MEDLINE | ID: mdl-20802941
INTRODUCTION: Isoenzymatic analyses were performed involving species of the Nyssorhynchus and Anopheles subgenera in order to estimate the intra and interspecies genetic variability. METHODS: Mosquitoes were caught at different localities in the Amazon region. The collection and rearing of mosquitoes in the laboratory followed specific protocols. For the genetic variability analyses, the technique of horizontal electrophoresis on starch and starch-agarose gel with appropriate buffer systems was used. The alloenzyme variation was estimated using the Biosys-1 software. RESULTS: Out of the 13 loci, eight were polymorphic. Anopheles nuneztovari presented the largest number of alleles per locus, while the smallest number was detected in Anopheles marajoara from Macapá. The largest number of polymorphic loci was found for Anopheles marajoara from Maruanum and the smallest for Anopheles benarrochi (Guayará Mirim). Anopheles darlingi (Macapá) presented the greatest heterozygosity (Ho = 0.167 +/- 0.071), while the lowest heterozygosity (Ho = 0.045 +/- 0.019) was observed in Anopheles intermedius (Pacoval) of the subgenus Anopheles. Wright's F coefficient revealed considerable genetic structuring between the populations of Anopheles darlingi (Fst = 0.110) and between the populations of Anopheles marajoara (Fst = 0.082). CONCLUSIONS: Considering all the species studied, the genetic distance ranged from 0.008 to 1.114. The greatest distance was between Anopheles mattogrossensis and Anopheles oswaldoi, while the smallest was between the Anopheles benarrochi populations.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Polimorfismo Genético / Variação Genética / Anopheles Limite: Animals País/Região como assunto: America do sul / Brasil Idioma: En Revista: Rev Soc Bras Med Trop Ano de publicação: 2010 Tipo de documento: Article País de afiliação: Brasil País de publicação: Brasil

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Polimorfismo Genético / Variação Genética / Anopheles Limite: Animals País/Região como assunto: America do sul / Brasil Idioma: En Revista: Rev Soc Bras Med Trop Ano de publicação: 2010 Tipo de documento: Article País de afiliação: Brasil País de publicação: Brasil