Calcitriol down-regulates human ether a go-go 1 potassium channel expression in cervical cancer cells.
Anticancer Res
; 30(7): 2667-72, 2010 Jul.
Article
em En
| MEDLINE
| ID: mdl-20682996
BACKGROUND/AIM: Human ether à-go-go-1 (EAG1) potassium channels are promising anticancer targets. Calcitriol has antitumoural properties. This study investigated EAG1 regulation by calcitriol in normal and cancer cells. MATERIALS AND METHODS: Cancer cell lines from cervix, prostate, mammary gland, and normal placenta trophoblasts were cultured. Calcitriol was determined by HPLC. Gene and protein expression were assessed by real-time RT-PCR and western blot analysis, respectively. Calcitriol-synthesising enzyme CYP27B1 or vitamin D receptor (VDR), were transfected in cervical cancer SiHa cells. Cell proliferation was assayed with XTT. RESULTS: Calcitriol decreased EAG1 mRNA in all cell types, and EAG1 protein and proliferation in SiHa cells. VDR antagonist ZK-159222 prevented the calcitriol effect on EAG1 mRNA. CYP27B1-transfected cells produced more calcitriol and less EAG1 mRNA. EAG1 mRNA was more potently inhibited by calcitriol in VDR-transfected cells. CONCLUSION: EAG1 is a calcitriol target in normal and cancer cells and calcitriol is a potential therapy for cervical cancer.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Calcitriol
/
Neoplasias do Colo do Útero
/
Canais de Potássio Éter-A-Go-Go
Limite:
Female
/
Humans
/
Male
Idioma:
En
Revista:
Anticancer Res
Ano de publicação:
2010
Tipo de documento:
Article
País de afiliação:
México
País de publicação:
Grécia