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Manipulating the molecular weight of alginate produced by Azotobacter vinelandii in continuous cultures.
Díaz-Barrera, Alvaro; Silva, Paulina; Berrios, Julio; Acevedo, Fernando.
Afiliação
  • Díaz-Barrera A; Escuela de Ingeniería Bioquímica, Pontificia Universidad Cato lica de Valparaíso, Av. Brasil 2147, Casilla 4059, Valparaíso, Chile. alvaro.diaz@ucv.cl
Bioresour Technol ; 101(23): 9405-8, 2010 Dec.
Article em En | MEDLINE | ID: mdl-20675122
Alginate production by Azotobacter vinelandii in chemostat cultures was evaluated at different dilution rates (D) and inlet sucrose concentrations of 5 and 20 g l(-1). At the low inlet sucrose concentration, the molecular weight of alginate increased from 800 to 1800 kDa when D increased from 0.05 to 0.10 h(-1), whereas the opposite trend was observed with the high inlet sucrose concentration. This behaviour can be explained by changes in specific sucrose uptake rate. Thus, a decrease in alginate molecular weight was dependent on the specific sucrose uptake rate when this rate was higher than 0.42 g g(-1) h(-1). The manipulation of the D can be used to select the molecular weight of alginate in continuous culture.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Azotobacter vinelandii / Técnicas de Cultura de Células / Alginatos Idioma: En Revista: Bioresour Technol Assunto da revista: ENGENHARIA BIOMEDICA Ano de publicação: 2010 Tipo de documento: Article País de afiliação: Chile País de publicação: Reino Unido

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Azotobacter vinelandii / Técnicas de Cultura de Células / Alginatos Idioma: En Revista: Bioresour Technol Assunto da revista: ENGENHARIA BIOMEDICA Ano de publicação: 2010 Tipo de documento: Article País de afiliação: Chile País de publicação: Reino Unido