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Quantification of fibrosis and mast cells in the tissue response of endodontic sealer irradiated by low-level laser therapy.
Berbert, Fábio Luiz Camargo Villela; Sivieri-Araújo, Gustavo; Ramalho, Lizeti Toledo Oliveira; Pereira, Sanívia Aparecida Lima; Rodrigues, Denise Bertulucci Rocha; de Araújo, Marcelo Sivieri.
Afiliação
  • Berbert FL; Department of Restorative Dentistry, Discipline of Endodontics, Dental School of Araraquara, São Paulo State University-UNESP, Rua Humaitá, 1680, PO 331, CEP 14.801-903, Araraquara, SP, Brazil.
Lasers Med Sci ; 26(6): 741-7, 2011 Nov.
Article em En | MEDLINE | ID: mdl-20549281
Low-level laser therapy (LLLT) accelerates tissue repair. Mast cells induce the proliferation of fibroblasts and the development of local fibrosis. The objective of this study was to quantify fibrosis rate and mast cells in connective tissue after endodontic sealer zinc oxide and eugenol (ZOE) was implanted and submitted to LLLT, immediately after implant and again 24 h later. Sixty mice were distributed into three groups: GI, GII, and GIII (n = 20). In GI, the tubes filled with Endofill were implanted in the animals and were not irradiated with LLLT. In GII, the tubes containing Endofill were implanted in the animals and then irradiated with red LLLT (InGaAIP) 685-nm wavelength, D = 72 J/Cm(2), E = 2 J, T = 58 s, P = 35 mW, and in GIII, the tubes with Endofill were implanted and irradiated with infrared LLLT (AsGaAl) 830-nm wavelength, D = 70 J/Cm(2), E = 2 J, T = 40 s, P = 50 mW. After 7 days and 30 days, the animals were killed. A series of 6-µm-thick sections were obtained and stained with Toluidine Blue and Picrosirius and analyzed under a standard light microscope using a polarized light filter for the quantification of fibrosis. The statistics were qualitative and quantitative with a significance of 5%. The irradiation with LLLT did not offer improvement in the fibrosis rate, however, it provided a significant decrease in the concentration of independent mast cells for the period studied.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Materiais Restauradores do Canal Radicular / Cicatrização / Terapia com Luz de Baixa Intensidade Tipo de estudo: Evaluation_studies / Qualitative_research Limite: Animals Idioma: En Revista: Lasers Med Sci Assunto da revista: BIOTECNOLOGIA / RADIOLOGIA Ano de publicação: 2011 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Materiais Restauradores do Canal Radicular / Cicatrização / Terapia com Luz de Baixa Intensidade Tipo de estudo: Evaluation_studies / Qualitative_research Limite: Animals Idioma: En Revista: Lasers Med Sci Assunto da revista: BIOTECNOLOGIA / RADIOLOGIA Ano de publicação: 2011 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido