[Understanding the pathophysiology of epilepsy in an animal model: pentylenetetrazole induces activation but not death of neurons of the medial extended amygdala]. / Descifrando la fisiopatología de la epilepsia en un modelo animal: el pentilentetrazol induce la activación pero no la muerte de las neuronas de la amígdala extendida medial.
Neurologia
; 25(3): 148-55, 2010 Apr.
Article
em Es
| MEDLINE
| ID: mdl-20492860
INTRODUCTION: since middle of the 20th century the importance of amygdala in epilepsy it has suggested, although the basic mechanisms of this participation are still unknown. This ignorance increases when the different subdivisions of amygdala are considered, especially the medial amygdala. In this work we assess the involvement of the medial extended amygdala in an animal model of epilepsy and the consequences of its application in this brain structure. MATERIAL AND METHODS: forty eight adult Wistar male rats were used, of which 24 of them received i.p. injections of pentylenetetrazole, and 24 (controls) were injected with saline. After 2, 6, 12 and 24 h survival, animals were fixed; the brains were sectioned serially and stained for fos (immunochemistry) and for neuronal death with the A-Cu-Ag technique. Data were analysed using two-way ANOVA followed by the Fisher post hoc test. RESULTS: very few or no fos-immunoreactive neurons were seen in control animals. In experimental animals, fos was rapidly induced in structures of medial extended amygdala with peak levels at 2 h. Marked fos immunoreactivity persisted up to 12 h followed by a gradual return to baseline at 24 h. However, status epilepticus did not induced neuronal death. CONCLUSIONS: these results show involvement of medial extended amygdala in epileptic mechanisms with an inhibitory component. However, neuronal death is not a consequence of status epilepticus-induced by pentylentetrazole.
Buscar no Google
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Pentilenotetrazol
/
Convulsivantes
/
Epilepsia
/
Tonsila do Cerebelo
/
Neurônios
Limite:
Animals
/
Humans
/
Male
Idioma:
Es
Revista:
Neurologia
Assunto da revista:
NEUROLOGIA
Ano de publicação:
2010
Tipo de documento:
Article
País de afiliação:
Argentina
País de publicação:
Espanha