[Effects of low-level helium-neon laser on induced wound healing in rats]. / Os efeitos do laser hélio-neônio de baixa intensidade na cicatrização de lesões cutâneas induzidas em ratos.
Rev Bras Fisioter
; 14(1): 45-51, 2010.
Article
em Pt
| MEDLINE
| ID: mdl-20414561
OBJECTIVE: To evaluate the effects of low-level helium-neon (HeNe) laser on cutaneous wound healing in rats. METHODS: Sixty Wistar rats were divided into control group and experimental group. A sutured longitudinal, dorsal-medial incision was made, with simple separate stitches. The experimental group was irradiated daily in three areas of the wound with HeNe laser (5mW maximum continuous power, 632.8 nm wavelength, 4 J/cm(2) energy density and 0.015 cm(2) laser beam area) for 36 seconds. The areas were evaluated on the third, seventh and fourteenth days postoperative. Histological sections were stained with hematoxylin-eosin to determine the type of inflammatory reaction according to the protocol by Vizzotto et al. (2003)* and with Picrosirius to identify types I and III collagen and the collagen maturation index (CMI). Immunohistochemical detection was employed with anti-CD45-LCA to recognize the inflammatory cells. RESULTS: Both groups had the same inflammatory pattern. The experimental group had fewer inflammatory cells at the three evaluation times (p<0.001) with faster reduction in the number of leukocytes. The experimental group had greater total collagen density on the third day (p=0.001), with more type III collagen (p=0.001) and more type I collagen (p=0.001). There was no significant difference in the CMI. CONCLUSION: Low-level laser irradiation does not change the quality of the inflammatory response, but it does reduce its intensity; it increases collagen deposition in the early stages of the healing process and does not interfere with collagen maturation.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Pele
/
Cicatrização
/
Lasers de Gás
Limite:
Animals
Idioma:
Pt
Revista:
Rev Bras Fisioter
Assunto da revista:
MEDICINA FISICA
/
REABILITACAO
Ano de publicação:
2010
Tipo de documento:
Article
País de afiliação:
Brasil
País de publicação:
Brasil