Complete genome amplification of equine influenza virus subtype 2.
Rev Argent Microbiol
; 41(4): 207-11, 2009.
Article
em En
| MEDLINE
| ID: mdl-20085182
This work reports a method for rapid amplification of the complete genome of equine influenza virus subtype 2 (H3N8). A ThermoScript reverse transcriptase instead of the avian myeloblastosis virus reverse transcriptase or Moloney murine leukemia virus reverse transcriptase was used. This enzyme has demonstrated higher thermal stability and is described as suitable to make long cDNA with a complex secondary structure. The product obtained by this method can be cloned, used in later sequencing reactions or nested-PCR with the purpose of achieving a rapid diagnosis and characterization of the equine influenza virus type A. This detection assay might be a valuable tool for diagnosis and screening of field samples as well as for conducting molecular studies.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Genoma Viral
/
Reação em Cadeia da Polimerase Via Transcriptase Reversa
/
Vírus da Influenza A Subtipo H3N8
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
Idioma:
En
Revista:
Rev Argent Microbiol
Ano de publicação:
2009
Tipo de documento:
Article
País de afiliação:
Argentina
País de publicação:
Argentina