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Infection kinetics of human adenovirus serotype 41 in HEK 293 cells.
Siqueira-Silva, Joselma; Yeda, Fernanda Perez; Favier, Anne-Laure; Mezin, Paulette; Silva, Misael Leonardo; Barrella, Karina Médici; Mehnert, Dolores Ursula; Fender, Pascal; Hársi, Charlotte Marianna.
Afiliação
  • Siqueira-Silva J; Laboratório de Biologia Molecular de Adenovírus, Departamento de Microbiologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, SP, Brazil.
Mem Inst Oswaldo Cruz ; 104(5): 736-44, 2009 Aug.
Article em En | MEDLINE | ID: mdl-19820835
The purpose of this work was to acquire an overview of the infectious cycle of HAdV-41 in permissive HEK 293 cells and compare it to that observed with the prototype of the genus, Human adenovirus C HAdV-2. HEK 293 cells were infected with each virus separately and were harvested every 12 h for seven days. Infection kinetics were analysed using confocal and electronic microscopy. The results show that, when properly cultivated, HAdV-41 was not fastidious. It had a longer multiplication cycle, which resulted in the release of complete viral particles and viral stocks reached high titres. After 60 h of infection, the export of viral proteins from the infected cell to the extracellular milieu was observed, with a pattern similar to that previously described for HAdV-2 penton-base trafficking after 30 h of infection. HAdV-41 had a non-lytic cycle and the infection spread from the first infected cell to its neighbours. The release process of the viral particles is unknown. The results observed for HAdV-41 infection in HEK 293 cells show how different this virus is from the prototype HAdV-2 and provides information for the development of this vector for use in gene therapy.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Adenovírus Humanos Limite: Animals / Humans Idioma: En Revista: Mem Inst Oswaldo Cruz Ano de publicação: 2009 Tipo de documento: Article País de afiliação: Brasil País de publicação: Brasil

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Adenovírus Humanos Limite: Animals / Humans Idioma: En Revista: Mem Inst Oswaldo Cruz Ano de publicação: 2009 Tipo de documento: Article País de afiliação: Brasil País de publicação: Brasil