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Retinoic acid inhibits dendritic cell differentiation driven by interleukin-4.
de Sousa-Canavez, Juliana Moreira; de Oliveira Massoco, Cristina; de Moraes-Vasconcelos, Dewton; Corneta, Elaine Cristina; Leite, Katia Ramos Moreira; Camara-Lopes, Luiz Heraldo.
Afiliação
  • de Sousa-Canavez JM; Genoa Biotechnology SA, Desembargador Eliseu de Almeida, 9th floor, São Paulo, SP, Brazil. jmscanavez@genoabiotec.com.br
Cell Immunol ; 259(1): 41-8, 2009.
Article em En | MEDLINE | ID: mdl-19540457
All-trans-retinoic acid (atRA) appears to affect Th1-Th2 differentiation and its effects on immune responses might also be mediated by dendritic cell (DC). Nonetheless, studies have been showing contradictory results since was observed either induction or inhibition of DC differentiation. Our aim was to investigate atRA action on human monocyte derived DC differentiation. For this purpose we tested pharmacological and physiological doses of atRA with or without cytokines. Cell phenotypes were analyzed by flow cytometry and function was investigated by phagocytosis and respiratory burst. DC, positive control group, was differentiated with GM-CSF and IL-4 and maturated with TNF-alpha. We demonstrated that atRA effects depend on the dose used as pharmacological doses inhibited expression of all phenotypic markers tested while a physiological dose caused cell differentiation. However, atRA combined or not with cytokines did not promote DC differentiation. In fact, atRA was detrimental on IL-4 property as a DC inductor.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Tretinoína / Células Dendríticas / Diferenciação Celular / Interleucina-4 Limite: Humans Idioma: En Revista: Cell Immunol Ano de publicação: 2009 Tipo de documento: Article País de afiliação: Brasil País de publicação: Holanda

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Tretinoína / Células Dendríticas / Diferenciação Celular / Interleucina-4 Limite: Humans Idioma: En Revista: Cell Immunol Ano de publicação: 2009 Tipo de documento: Article País de afiliação: Brasil País de publicação: Holanda