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Determination of 2,5-hexanedione in urine by headspace solid-phase microextraction and gas chromatography.
Oliveira, Antônio Felipe F; Maia, Patrícia P; Paiva, Maria José N; Siqueira, Maria Elisa P B.
Afiliação
  • Oliveira AF; Laboratory of Toxicological Analysis, Federal University of Alfenas, 37130-000 Alfenas, MG, Brazil.
J Anal Toxicol ; 33(4): 223-8, 2009 May.
Article em En | MEDLINE | ID: mdl-19470226
2,5-Hexanedione (2,5-HD) is the most important metabolite of n-hexane and methyl ethyl ketone in human urine. Urinary 2,5-HD is used as a biomarker for biological monitoring of workers exposed to n-hexane. A simple method using headspace solid-phase microextraction (HS-SPME) and gas chromatography (GC) equipped with a flame-ionization detector (FID) was developed. The parameters that affect the HS-SPME-GC-FID process were optimized (i.e., fiber coating, sample volume, adsorption and heating time, salt addition, and extraction temperature). The assay presented linearity in the range of 0.075 to 20.0 mg/L, precision (coefficient of variation < 7.0%), and detection limit of 0.025 mg/L for 2,5-HD in urine. The method was successfully applied to the analysis of 2,5-HD in urine samples from eight workers occupationally exposed to n-hexane in shoemaker's glue.
Assuntos
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Adesivos / Monitoramento Ambiental / Cromatografia Gasosa / Exposição Ocupacional / Microextração em Fase Sólida / Hexanos / Hexanonas Limite: Humans Idioma: En Revista: J Anal Toxicol Ano de publicação: 2009 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Adesivos / Monitoramento Ambiental / Cromatografia Gasosa / Exposição Ocupacional / Microextração em Fase Sólida / Hexanos / Hexanonas Limite: Humans Idioma: En Revista: J Anal Toxicol Ano de publicação: 2009 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido