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Differential response of antigen presenting cells from susceptible and resistant strains of mice to Taenia crassiceps infection.
Reyes, José L; Terrazas, César A; Vera-Arias, Laura; Terrazas, Luis I.
Afiliação
  • Reyes JL; Unidad de Biomedicina, Facultad de Estudios Superiores-Iztacala, Universidad Nacional Autónoma de México, Mexico.
Infect Genet Evol ; 9(6): 1115-27, 2009 Dec.
Article em En | MEDLINE | ID: mdl-19465163
Antigen presenting cells (APCs) are critically involved in the interaction between pathogens and the host immune system. Here, we examined two different populations of APCs in mice that are susceptible (BALB/c) or resistant (C57BL/6) to Taenia crassiceps cysticercosis. Bone marrow-derived dendritic cells (BMDCs) from both strains of mice were exposed to T. crassiceps excreted/secreted antigens (TcES) and, at the same time, to the Toll-like receptor (TLR) ligand LPS. BMDCs from BALB/c mice underwent a partial maturation when incubated with TcES and displayed decreased responses to TLR-dependent stimuli associated with low CD80, CD86, CD40 and CCR7 expression and impaired IL-15 production. These BMDCs-induced impaired allogenic responses. In contrast, BMDCs from C57BL/6 mice displayed normal maturation and induced strong allogenic responses. Moreover, the exposure to TcES resulted in a lower production of IL-12 and TNF-alpha by LPS-activated DCs from BALB/c mice compared to C57BL/6 DCs. Three parameters of macrophage activation were assessed during Taenia infection: LPS+IFN-gamma-induced production of IL-12, TNF-alpha and nitric oxide (NO) in vitro; infection-induced markers for alternatively activated macrophages (Arginase-1, RELM-alpha, Ym-1 and TREM-2 expression) and suppressive activity. The maximum response to LPS+IFN-gamma-induced TNF-alpha, IL-12 and NO production by macrophages from both strains of mice occurred 2 wk post-infection. However, as infection progressed, the production of these molecules by BALB/c macrophages declined. While the BALB/c macrophages displayed impaired pro-inflammatory responses, these macrophages showed strong Arginase-1, Ym-1, RELM-alpha and TREM-2 expression. By contrast, C57BL/6 macrophages maintained a pro-inflammatory profile and low transcripts for alternative activation markers. Macrophages from T. crassiceps-infected BALB/c mice showed stronger suppressive activity than those from C57BL/6 mice. These findings suggest that APC activation at both early and late time points during T. crassiceps infection is a possible mechanism that underlies the differential susceptibility to T. crassiceps infection displayed by these mouse strains.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Taenia / Teníase / Células Dendríticas / Predisposição Genética para Doença / Macrófagos Limite: Animals Idioma: En Revista: Infect Genet Evol Assunto da revista: BIOLOGIA / DOENCAS TRANSMISSIVEIS / GENETICA Ano de publicação: 2009 Tipo de documento: Article País de afiliação: México País de publicação: Holanda

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Taenia / Teníase / Células Dendríticas / Predisposição Genética para Doença / Macrófagos Limite: Animals Idioma: En Revista: Infect Genet Evol Assunto da revista: BIOLOGIA / DOENCAS TRANSMISSIVEIS / GENETICA Ano de publicação: 2009 Tipo de documento: Article País de afiliação: México País de publicação: Holanda