The guinea pig as an animal model for Ipomoea carnea induced alpha-mannosidosis.
Toxicon
; 54(3): 276-82, 2009 Sep 01.
Article
em En
| MEDLINE
| ID: mdl-19393259
The toxic effects of Ipomoea carnea subsp. fistulosa were evaluated in guinea pigs by administration of dry leaves during 45 days. Swainsonine and calystegines B(1), B(2) and C(1) were isolated and quantified. Clinical signs included emaciated and loss of body weight. Histological evaluation demonstrates numerous vacuoles in the cytoplasm of pancreas, liver and renal cells. Vacuolation was also evident in neurons of brain stem, mainly pontine nuclei. Neuronal lectin binding pattern showed a strong positive reaction to Con-A (Concanavalia ensiformis), WGA (Triticum vulgaris), sWGA (succinylated T. vulgaris) and LCA (Lens culinary). This result is coincident with the lectin histochemistry staining pattern of the vacuoles described in CNS of ruminants. We conclude that I. carnea subsp. fistulosa induces an intralysosomal accumulation of mannose-containing oligosaccharides in guinea pigs, which makes it a valuable animal model for the reproduction of induced alpha-mannosidosis.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Ipomoea
/
Modelos Animais de Doenças
/
Alfa-Manosidose
Limite:
Animals
Idioma:
En
Revista:
Toxicon
Ano de publicação:
2009
Tipo de documento:
Article
País de afiliação:
Argentina
País de publicação:
Reino Unido