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Detection and characterization of group C rotavirus in Buenos Aires, Argentina, 1997-2003.
Castello, Alejandro A; Argüelles, Marcelo H; Rota, Rosana P; Humphrey, Charles D; Olthoff, Alicia; Gentsch, Jon R; Glass, Roger I; Glikmann, Graciela; Jiang, Baoming.
Afiliação
  • Castello AA; Division of Viral Diseases, Centers for Disease Control and Prevention, US Department of Health and Human Services, Atlanta, Georgia, USA. acastello@unq.edu.ar
J Med Virol ; 81(6): 1109-16, 2009 Jun.
Article em En | MEDLINE | ID: mdl-19382268
The role of group C rotaviruses as a cause of diarrhea was examined among children <17 years of age admitted to a Hospital in a suburban area of Buenos Aires, Argentina between 1997 and 2003. A total of 1,579 fecal samples were screened for group A (RVA) and C (RVC) rotaviruses by two in-house ELISA methods at Quilmes University (UNQ-ELISA). Samples positive, doubtful and negative by RVC specific UNQ-ELISA (n = 246) were examined further for RVC by another in-house ELISA (CDC-ELISA), electron microscopy, RT-PCR, nested PCR, and Southern hybridization. Sensitivity, specificity, and predictive values for each test were determined. While the sensitivity was comparable for the nested PCR and CDC-ELISA methods (82.5%), the molecular methods were slightly more specific. Poorly preserved particles were often seen in fecal samples, suggesting that degradation of RNA could be a factor influencing the performance of molecular methods. The incidence of RVC was estimated to be 3% without apparent differences among seasons. RVC infected patients had a significantly (P < 0.001) higher median age (6 years vs. 1 year) than those with RVA infection. Sequence of the RVC VP7 gene from six Argentinean strains and sequences reported previously in different countries showed high nucleotide (94.4-99.9%) sequence identities, indicating a high degree of conservation for human RVC VP7 genes among strains collected on five continents over a period of 17 years. These findings indicate that RVC is a significant cause of diarrhea and it is necessary to develop simple and sensitive serological methods for its detection.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Infecções por Rotavirus / Rotavirus / Diarreia Tipo de estudo: Diagnostic_studies / Evaluation_studies / Prognostic_studies Limite: Adolescent / Child / Child, preschool / Humans / Infant / Newborn País/Região como assunto: America do sul / Argentina Idioma: En Revista: J Med Virol Ano de publicação: 2009 Tipo de documento: Article País de afiliação: Estados Unidos País de publicação: Estados Unidos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Infecções por Rotavirus / Rotavirus / Diarreia Tipo de estudo: Diagnostic_studies / Evaluation_studies / Prognostic_studies Limite: Adolescent / Child / Child, preschool / Humans / Infant / Newborn País/Região como assunto: America do sul / Argentina Idioma: En Revista: J Med Virol Ano de publicação: 2009 Tipo de documento: Article País de afiliação: Estados Unidos País de publicação: Estados Unidos