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Prevalence of diarrhoeagenic Escherichia coli in children from León, Nicaragua.
Vilchez, Samuel; Reyes, Daniel; Paniagua, Margarita; Bucardo, Filemon; Möllby, Roland; Weintraub, Andrej.
Afiliação
  • Vilchez S; Division of Clinical Microbiology, Department of Laboratory Medicine, Karolinska Institutet, Karolinska University Hospital, S-141 86 Stockholm, Sweden.
  • Reyes D; Department of Microbiology, Faculty of Medical Sciences, National Autonomous University of Nicaragua (UNAN), León, Nicaragua.
  • Paniagua M; Department of Microbiology, Tumor and Cell Biology (MTC), Karolinska Institutet, S-171 77 Solna, Stockholm, Sweden.
  • Bucardo F; Department of Microbiology, Faculty of Medical Sciences, National Autonomous University of Nicaragua (UNAN), León, Nicaragua.
  • Möllby R; Department of Microbiology, Tumor and Cell Biology (MTC), Karolinska Institutet, S-171 77 Solna, Stockholm, Sweden.
  • Weintraub A; Department of Microbiology, Faculty of Medical Sciences, National Autonomous University of Nicaragua (UNAN), León, Nicaragua.
J Med Microbiol ; 58(Pt 5): 630-637, 2009 May.
Article em En | MEDLINE | ID: mdl-19369525
Diarrhoeal disease is a public health problem worldwide, mostly affecting children in developing countries. In Nicaragua, diarrhoea is the second greatest cause of infant mortality. During the period March 2005 to September 2006, a total of 526 faecal samples from children aged 0-60 months (381 with and 145 without diarrhoea) from León, Nicaragua, were studied. In order to detect five different diarrhoeagenic Escherichia coli pathotypes simultaneously [enterotoxigenic E. coli (ETEC), enteroaggregative E. coli (EAEC), enteropathogenic E. coli (EPEC), enterohaemorrhagic E. coli (EHEC) and enteroinvasive E. coli (EIEC)], a mixture of eight primer pairs was used in a single PCR. At least one diarrhoeagenic E. coli pathotype was detected in 205 samples (53.8%) of the diarrhoea group and in 77 samples (53.1%) in the non-diarrhoea group. ETEC was detected significantly more often in children with diarrhoea (20.5%) than in children without diarrhoea (8.3%) (P=0.001). Atypical EPEC, EIEC and EAEC were detected with slightly lower frequencies in children with (16.0, 0.8 and 27.8%, respectively) than in children without (20.7, 1.4 and 33.1%, respectively) diarrhoea. EHEC was only detected in children with diarrhoea (2.1%). In conclusion, ETEC continues to be an important agent associated with diarrhoea in children from León, Nicaragua. Although not very frequent, the only findings that were 100% associated with diarrhoea were ETEC estA (4.7%) and EHEC (2.1%). Nevertheless, EAEC and EPEC were also frequent pathotypes in the population under study. In children with severe diarrhoea, more than half had EAEC, ETEC or EPEC, and EAEC was the most prevalent pathotype.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Diarreia / Escherichia coli / Infecções por Escherichia coli Tipo de estudo: Prevalence_studies Limite: Child, preschool / Humans / Infant País/Região como assunto: America central / Nicaragua Idioma: En Revista: J Med Microbiol Ano de publicação: 2009 Tipo de documento: Article País de afiliação: Suécia País de publicação: Reino Unido
Texto completo
Adicionar na Minha BVS
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PubMed Links
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Diarreia / Escherichia coli / Infecções por Escherichia coli Tipo de estudo: Prevalence_studies Limite: Child, preschool / Humans / Infant País/Região como assunto: America central / Nicaragua Idioma: En Revista: J Med Microbiol Ano de publicação: 2009 Tipo de documento: Article País de afiliação: Suécia País de publicação: Reino Unido