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Determination of streptomycin residues in honey by liquid chromatography-tandem mass spectrometry.
Granja, Rodrigo H M M; Niño, Alfredo M Montes; Zucchetti, Roberto A M; Niño, Rosario E Montes; Patel, Raj; Salerno, Alessandro G.
Afiliação
  • Granja RH; Microbioticos Laboratories, Av. Santa Isabel 2120, Campinas, SP, 13084-471, Brazil.
Anal Chim Acta ; 637(1-2): 64-7, 2009 Apr 01.
Article em En | MEDLINE | ID: mdl-19286013
Streptomycin is an aminoglycoside antibiotic used in apiculture to protect bees against a variety of brood diseases. Brazilian authorities have included it in the National Regulatory Monitoring Program for honey production. A simple and reliable method using liquid chromatography-tandem mass spectrometry has been developed and validated for the determination of streptomycin in honey. The chromatography separation was performed on a Gemini 5 microm C18 (50 mm x 2 mm) column using 5mM heptafluorbutiric acid/acetonitrile (85:15) as the mobile phase at a flow rate of 0.2 mLmin(-1). The detection of the analyte was achieved by positive ionization electrospray in multiple reaction-monitoring modes. Two characteristic transitions were monitored for streptomycin. Some analytical parameters were validated according to the guidelines laid down by European Commission Decision 2002/657/EC: decision limit, detection capability, recovery, precision and ruggedness. The recoveries of streptomycin from honey fortified at 2.5, 10, 15 and 20 microgkg(-1) levels are around 100%. The decision limit and detection capability of streptomycin was 3 microgkg(-1) and 4.7 microgkg(-1) respectively.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Resíduos de Drogas / Estreptomicina / Cromatografia Líquida de Alta Pressão / Espectrometria de Massas em Tandem / Mel / Antibacterianos Tipo de estudo: Prognostic_studies Idioma: En Revista: Anal Chim Acta Ano de publicação: 2009 Tipo de documento: Article País de afiliação: Brasil País de publicação: Holanda

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Resíduos de Drogas / Estreptomicina / Cromatografia Líquida de Alta Pressão / Espectrometria de Massas em Tandem / Mel / Antibacterianos Tipo de estudo: Prognostic_studies Idioma: En Revista: Anal Chim Acta Ano de publicação: 2009 Tipo de documento: Article País de afiliação: Brasil País de publicação: Holanda