Possible role of NFkappaB in the embryonic vascular remodeling and the endothelial mesenchymal transition process.
Cell Adh Migr
; 2(1): 17-29, 2008.
Article
em En
| MEDLINE
| ID: mdl-19262121
The NFkappaB family of transcription factors, particularly the activated p50/p65 heterodimer, is expressed in vascular cells during intimal thickening formation when hemodynamic conditions are altered. Here, we report that p50, p65, IkappaBalpha and IKKalpha display different spatial and temporal patterns of expression and distribution during both chicken embryo aortic wall remodeling and intimal thickening development. Additionally, we show that both p50 and p65 were located in the nucleus of some mesenchymal cells expressing alpha-smooth muscle actin which are present in the spontaneous intimal thickening observed at embryonic days 12-14 of development. We also demonstrated that both NFkappaB subunits are present in monolayers of primary embryonic aortic endothelial cells attached to fibronectin and stimulated with complete medium. This study demonstrates for the first time the presence of activated NFkappaB during the remodeling of the embryonic aortic wall and the formation of intimal thickening, providing evidence that suggest a possible role for this transcription factor in the EndoMT process.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Aorta
/
Diferenciação Celular
/
NF-kappa B
/
Neovascularização Fisiológica
/
Células Endoteliais
/
Mesoderma
Limite:
Animals
Idioma:
En
Revista:
Cell Adh Migr
Ano de publicação:
2008
Tipo de documento:
Article
País de afiliação:
Venezuela
País de publicação:
Estados Unidos