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Prion protein and its ligand stress inducible protein 1 regulate astrocyte development.
Arantes, Camila; Nomizo, Regina; Lopes, Marilene H; Hajj, Glaucia N M; Lima, Flavia R S; Martins, Vilma R.
Afiliação
  • Arantes C; Ludwig Institute for Cancer Research, Hospital Alemão Oswaldo Cruz, São Paulo, Brazil.
Glia ; 57(13): 1439-49, 2009 Oct.
Article em En | MEDLINE | ID: mdl-19243016
Prion protein (PrP(C)) interaction with stress inducible protein 1 (STI1) mediates neuronal survival and differentiation. However, the function of PrP(C) in astrocytes has not been approached. In this study, we show that STI1 prevents cell death in wild-type astrocytes in a protein kinase A-dependent manner, whereas PrP(C)-null astrocytes were not affected by STI1 treatment. At embryonic day 17, cultured astrocytes and brain extracts derived from PrP(C)-null mice showed a reduced expression of glial fibrillary acidic protein (GFAP) and increased vimentin and nestin expression when compared with wild-type, suggesting a slower rate of astrocyte maturation in PrP(C)-null animals. Furthermore, PrP(C)-null astrocytes treated with STI1 did not differentiate from a flat to a process-bearing morphology, as did wild-type astrocytes. Remarkably, STI1 inhibited proliferation of both wild-type and PrP(C)-null astrocytes in a protein kinase C-dependent manner. Taken together, our data show that PrP(C) and STI1 are essential to astrocyte development and act through distinct signaling pathways.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Diferenciação Celular / Astrócitos / Proteínas PrPC / Proliferação de Células / Proteínas de Choque Térmico Limite: Animals Idioma: En Revista: Glia Assunto da revista: NEUROLOGIA Ano de publicação: 2009 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Diferenciação Celular / Astrócitos / Proteínas PrPC / Proliferação de Células / Proteínas de Choque Térmico Limite: Animals Idioma: En Revista: Glia Assunto da revista: NEUROLOGIA Ano de publicação: 2009 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos