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Can established cultured papilloma cells harbor bovine papillomavirus?
Campos, S R C; Trindade, C; Ferraz, O P; Giovanni, D N S; Lima, A A; Caetano, H V A; Carvalho, R F; Birgel, E H; Dagli, M L Z; Mori, E; Brandão, P E; Richtzenhain, L J; Beçak, W; Stocco, R C.
Afiliação
  • Campos SR; Laboratório de Genética, Instituto Butantan, São Paulo, SP, Brasil.
Genet Mol Res ; 7(4): 1119-26, 2008 Oct 21.
Article em En | MEDLINE | ID: mdl-19048490
Papillomaviruses have been reported to be very difficult to grow in cell culture. Also, there are no descriptions of cell cultures from lesions of bovine cutaneous papillomatosis, with identification of different bovine papilloma virus (BPV) DNA sequences. In the present report, we describe primary cell cultures from samples of cutaneous lesions (warts). We investigated the simultaneous presence of different BPV DNA sequences, comparing the original lesion to different passages of the cell cultures and to peripheral blood. BPV 1, 2 and 4 DNA sequences were found in lesion samples, and respective cell cultures and peripheral blood, supporting our previous hypothesis of the possible activity of these sequences in different samples and now also showing how they can be maintained in different passages of cell cultures.
Assuntos
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Papiloma / Verrugas / Doenças dos Bovinos / Papillomavirus Bovino 1 Limite: Animals Idioma: En Revista: Genet Mol Res Assunto da revista: BIOLOGIA MOLECULAR / GENETICA Ano de publicação: 2008 Tipo de documento: Article País de afiliação: Brasil País de publicação: Brasil
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Papiloma / Verrugas / Doenças dos Bovinos / Papillomavirus Bovino 1 Limite: Animals Idioma: En Revista: Genet Mol Res Assunto da revista: BIOLOGIA MOLECULAR / GENETICA Ano de publicação: 2008 Tipo de documento: Article País de afiliação: Brasil País de publicação: Brasil