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Reduction of prion infectivity in packed red blood cells.
Morales, Rodrigo; Buytaert-Hoefen, Kimberley A; Gonzalez-Romero, Dennisse; Castilla, Joaquin; Hansen, Eric T; Hlavinka, Dennis; Goodrich, Raymond P; Soto, Claudio.
Afiliação
  • Morales R; Department of Neurology, University of Texas Medical Branch, 301 University Boulevard, Galveston, TX 77555, USA; Facultad de Ciencias, Universidad de Chile, Santiago, Chile.
  • Buytaert-Hoefen KA; CaridianBCT Biotechnologies, L.L.C., Lakewood, CO, USA.
  • Gonzalez-Romero D; Department of Neurology, University of Texas Medical Branch, 301 University Boulevard, Galveston, TX 77555, USA.
  • Castilla J; Department of Neurology, University of Texas Medical Branch, 301 University Boulevard, Galveston, TX 77555, USA.
  • Hansen ET; CaridianBCT Biotechnologies, L.L.C., Lakewood, CO, USA.
  • Hlavinka D; CaridianBCT Biotechnologies, L.L.C., Lakewood, CO, USA.
  • Goodrich RP; CaridianBCT Biotechnologies, L.L.C., Lakewood, CO, USA.
  • Soto C; Department of Neurology, University of Texas Medical Branch, 301 University Boulevard, Galveston, TX 77555, USA. Electronic address: clsoto@utmb.edu.
Biochem Biophys Res Commun ; 377(2): 373-378, 2008 Dec 12.
Article em En | MEDLINE | ID: mdl-18851948
The link between a new variant form of Creutzfeldt-Jakob disease (vCJD) and the consumption of prion contaminated cattle meat as well as recent findings showing that vCJD can be transmitted by blood transfusion have raised public health concerns. Currently, a reliable test to identify prions in blood samples is not available. The purpose of this study was to evaluate the possibility to remove scrapie prion protein (PrP(Sc)) and infectivity from red blood cell (RBC) suspensions by a simple washing procedure using a cell separation and washing device. The extent of prion removal was assessed by Western blot, PMCA and infectivity bioassays. Our results revealed a substantial removal of infectious prions (3 logs of infectivity) by all techniques used. These data suggest that a significant amount of infectivity present in RBC preparations can be removed by a simple washing procedure. This technology may lead to increased safety of blood products and reduce the risk of further propagation of prion diseases.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Transfusão de Sangue / Separação Celular / Síndrome de Creutzfeldt-Jakob / Proteínas PrPSc / Eritrócitos Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Biochem Biophys Res Commun Ano de publicação: 2008 Tipo de documento: Article País de afiliação: Chile País de publicação: Estados Unidos
Texto completo
Adicionar na Minha BVS
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XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Transfusão de Sangue / Separação Celular / Síndrome de Creutzfeldt-Jakob / Proteínas PrPSc / Eritrócitos Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Biochem Biophys Res Commun Ano de publicação: 2008 Tipo de documento: Article País de afiliação: Chile País de publicação: Estados Unidos