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Typing of four genetic loci discriminates among closely related species of New World Leishmania.
Tsukayama, Pablo; Lucas, Carmen; Bacon, David J.
Afiliação
  • Tsukayama P; Parasitology Program, Naval Medical Research Center Detachment, Lima, Peru.
Int J Parasitol ; 39(3): 355-62, 2009 Feb.
Article em En | MEDLINE | ID: mdl-18817779
All New World Leishmania species can cause cutaneous lesions, while only Leishmania (Viannia) braziliensis has been associated with mucosal metastases. Multilocus enzyme electrophoresis (MLEE) is the optimal standard for species identification but is slow and costly. New methods for species identification are needed to ensure proper identification and therapy. The coding regions of four metabolic enzyme markers in the MLEE typing method: mannose phosphate isomerase (MPI), malate dehydrogenase (MDH), glucose-6-phosphate isomerase (GPI), and 6-phosphogluconate dehydrogenase (6PGD), were analysed from seven species of New World Leishmania isolated from patients with either cutaneous or mucosal lesions to identify specific genetic polymorphisms responsible for the phenotypic variations observed in the MLEE typing scheme. We identified species-specific polymorphisms and determined that a combination of sequencing of the mpi and 6pgd genes was sufficient to differentiate among seven closely related species of New World Leishmania and among isolates of L. braziliensis shown previously to have atypical MLEE patterns. When DNA isolated from 10 cutaneous lesion biopsies were evaluated, the sequence typing method was 100% concordant with the published MLEE/monoclonal antibody identification methods. The identification of species-specific polymorphisms can be used to design a DNA-based test with greater discriminatory power that requires shorter identification times. When the causative agent of the disease is L. braziliensis, this method ensures correct species identification, even when the agent is a genetic variant. Proper identification could facilitate adequate treatment, preventing the onset of the disfiguring mucosal form of the disease.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Leishmaniose Cutânea / Loci Gênicos / Leishmania Tipo de estudo: Prognostic_studies Limite: Animals / Humans País/Região como assunto: America central / America do sul Idioma: En Revista: Int J Parasitol Ano de publicação: 2009 Tipo de documento: Article País de afiliação: Peru País de publicação: Reino Unido

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Leishmaniose Cutânea / Loci Gênicos / Leishmania Tipo de estudo: Prognostic_studies Limite: Animals / Humans País/Região como assunto: America central / America do sul Idioma: En Revista: Int J Parasitol Ano de publicação: 2009 Tipo de documento: Article País de afiliação: Peru País de publicação: Reino Unido