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Leukocyte response and phagocytic activity in Nile tilapia experimentally infected with Enterococcus sp.
Martins, Maurício L; Vieira, Felipe N; Jerônimo, Gabriela T; Mouriño, José L P; Dotta, Geovana; Speck, Gisele M; Bezerra, Adolfo J M; Pedrotti, Fabíola S; Buglione-Neto, Celso C; Pereira, Gilberto.
Afiliação
  • Martins ML; Aquaculture Department, Laboratory of Diagnosis and Pathology in Aquaculture, Federal University of Santa Catarina, Florianópolis, SC, Brazil. mlaterca@cca.ufsc.br
Fish Physiol Biochem ; 35(1): 219-22, 2009 Mar.
Article em En | MEDLINE | ID: mdl-18777139
This study evaluated the total and differential leukocyte counting and the phagocytic activity in Nile tilapia Oreochromis niloticus experimentally injected with Enterococcus sp. in the swim bladder. Fish were distributed in four treatments in triplicates of non-injected fish, fish injected with 1 ml of sterile saline solution 0.65%, and fish injected with 1 x 10(3) and 1 x 10(6) colony-forming units (CFU) of Enterococcus diluted in 1 ml sterile saline. Twenty-four hours after injection, the fish were anesthetized and the blood collected for white blood cell (WBC) counts, differential counting of WBC, and phagocytic activity of blood leukocytes. The increased numbers of WBC and lymphocytes were followed by decreased number of monocyte after infection. The percentages of phagocytic activities in the blood were 55.3 and 55.9%, respectively, in tilapia injected with 1 x 10(3) and 1 x 10(6) CFU/ml.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Fagocitose / Infecções por Bactérias Gram-Positivas / Enterococcus / Ciclídeos / Doenças dos Peixes / Leucócitos Limite: Animals Idioma: En Revista: Fish Physiol Biochem Ano de publicação: 2009 Tipo de documento: Article País de afiliação: Brasil País de publicação: Holanda

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Fagocitose / Infecções por Bactérias Gram-Positivas / Enterococcus / Ciclídeos / Doenças dos Peixes / Leucócitos Limite: Animals Idioma: En Revista: Fish Physiol Biochem Ano de publicação: 2009 Tipo de documento: Article País de afiliação: Brasil País de publicação: Holanda