Kinetics of the reduction of oxaloacetate catalyzed by mitochondrial malate dehydrogenase of Toxocara canis muscle.

Mansini, E; Oestreicher, E G; Ribeiro, L P

Mansini, E; Oestreicher, E G; Ribeiro, L P.

Afiliação

Mansini E; Departamento de Bioquimica, Universidade Federal do Rio de Janeiro, Brasil.

Comp Biochem Physiol B ; 98(2-3): 333-7, 1991.

Article em En | MEDLINE | ID: mdl-1873988

RESUMO

1. The reaction of reduction of oxaloacetate to L-malate in the presence of NADH catalyzed by mitochondrial malate dehydrogenase (EC 1.1.1.37) of Toxocara canis muscle has been studied. 2. The data obtained in initial velocity experiments as well as those involving product inhibition suggest that the reaction mechanism is of the sequential type with a kinetically significant ternary complex and in which the coenzymes bind to the free enzyme. 3. The kinetic parameters, including the inhibition constant for NADH were estimated by non-linear regression analysis using the appropriate rate equations.

Assuntos

Malato Desidrogenase/metabolismo; Mitocôndrias Musculares/enzimologia; Oxaloacetatos/metabolismo; Toxocara/enzimologia; Animais; Cães; Cinética; Malatos/metabolismo; NAD/metabolismo; Oxirredução

Buscar no Google

Adicionar na Minha BVS

Imprimir

XML

PubMed Links

Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Oxaloacetatos / Toxocara / Malato Desidrogenase / Mitocôndrias Musculares Limite: Animals Idioma: En Revista: Comp Biochem Physiol B Ano de publicação: 1991 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido

Buscar no Google

Adicionar na Minha BVS

Imprimir

XML

PubMed Links