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A synthetic peptide derived from alpha-fetoprotein inhibits the estradiol-induced proliferation of mammary tumor cells in culture through the modulation of p21.
Sierralta, Walter D; Epuñan, María J; Reyes, José M; Valladares, Luis E; Pino, Ana M.
Afiliação
  • Sierralta WD; INTA-Universidad de Chile, Santiago, Chile.
Adv Exp Med Biol ; 617: 463-8, 2008.
Article em En | MEDLINE | ID: mdl-18497070
A stable cyclized 9-mer peptide (cP) containing the active site of alpha-alpha fetoprotein (alphaFP) has been shown to be effective for prevention of estrogen-stimulated tumor cell proliferation in culture or of xenographt growth in immunodeficient mice. cP does not block 17beta-estradiol (E2) binding to its receptors, but rather appears to interfere with intracellular processing of the signal that supports growth. To obtain insight on that mechanism we studied the effect of cP on the proliferation of MCF-7 cells in culture. Proliferation in the presence of 2 microM E2 is decreased up to 40% upon addition of 2 microg ml(-1) cP to the medium; the presence of cP did not increase cell death, cP reduced also the proliferation of estrogen-dependent ZR75-1 cells but had no effect on autonomous MDA-MB-231 cells, cP did not modify the number of binding sites for labeled E2 or affected cell death. We detected increased nuclear p21Cip1 immunoreactivity after cP treatment. Our results suggest that cP acts via p21Cip1 to slow the process of MCF-7 cells through the cycle.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Peptídeos Cíclicos / Neoplasias da Mama / Alfa-Fetoproteínas / Proliferação de Células / Estradiol / Inibidor de Quinase Dependente de Ciclina p21 Limite: Animals / Female / Humans Idioma: En Revista: Adv Exp Med Biol Ano de publicação: 2008 Tipo de documento: Article País de afiliação: Chile País de publicação: Estados Unidos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Peptídeos Cíclicos / Neoplasias da Mama / Alfa-Fetoproteínas / Proliferação de Células / Estradiol / Inibidor de Quinase Dependente de Ciclina p21 Limite: Animals / Female / Humans Idioma: En Revista: Adv Exp Med Biol Ano de publicação: 2008 Tipo de documento: Article País de afiliação: Chile País de publicação: Estados Unidos