[Isolation, purification and characterization of a lectin from the seed of Erythrina costaricensis (Leguminosae)]. / Aislamiento, purificación y caracterización de una lectina de la semilla del poró, Erythrina costaricensis (Leguminosae).
Rev Biol Trop
; 39(1): 15-21, 1991 Jun.
Article
em Es
| MEDLINE
| ID: mdl-1844149
A lectin was isolated from the crude extract prepared from the seeds of E. costaricensis. It was purified by gel filtration on Sephadex G-100 followed by DEAE-Sephadex A-50 chromatography. PAGE revealed only one protein band, while analytical isoelectric focusing revealed four bands. The protein is a dimer with M(r) 58kda not united by disulfide bridges. It is a glycoprotein with 6.5% of neutral sugars, stable at 70 degrees C and at a pH range between 2 to 10. The protein exhibited a non specific agglutination of human erythrocytes, nevertheless it differentiated between erythrocytes of animal origin, agglutinating those of rabbit and chicken and not those from horse, goat, sheep or rat. Galactose, N-acetyl-D-galactosamine, lactose and EDTA are inhibitors while Ca++ and Mn++ acted as activators of the agglutination. No change in the blood pressure was observed when the lectin was intravenously injected in rats.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Plantas Medicinais
/
Erythrina
/
Lectinas
Limite:
Animals
/
Humans
Idioma:
Es
Revista:
Rev Biol Trop
Ano de publicação:
1991
Tipo de documento:
Article
País de publicação:
Costa Rica