Stage-specific expression of Trypanosoma cruzi trans-sialidase involves highly conserved 3' untranslated regions.
FEMS Microbiol Lett
; 283(2): 182-8, 2008 Jun.
Article
em En
| MEDLINE
| ID: mdl-18422619
trans-Sialidases (TSs) are virulence factors that allow some trypanosomatids to incorporate sialic acid from host molecules. Trypanosoma cruzi bears a complex gene family coding for TS members, which can be broadly divided into two groups: one translated in stages present in the mammalian host (trypomastigote TS, tTS) and one translated in the insect vector stages (epimastigote TS, eTS). The molecular basis underlying the expression of different, nonoverlapping sets of TS proteins in either host is poorly understood, particularly because of the lack of transcription initiation control in this organism. Here we show that 3' untranslated regions (3'UTRs) of tTS and eTS are highly conserved within each gene group but completely different between both groups. Importantly, tTS-3'UTR but not eTS-3'UTR promoted high expression of the green fluorescent protein reporter gene in the mammalian-dwelling stages. In epimastigotes, both 3'UTRs lead to a comparatively low expression of the reporter gene, although eTS-3'UTR was more efficient than tTS-3'UTR. These results stress the importance of posttranscriptional events, mainly driven by specific 3'UTRs, in gene expression regulation in T. cruzi.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Trypanosoma cruzi
/
Glicoproteínas
/
Regulação da Expressão Gênica
/
Regiões 3' não Traduzidas
/
Neuraminidase
Limite:
Animals
Idioma:
En
Revista:
FEMS Microbiol Lett
Ano de publicação:
2008
Tipo de documento:
Article
País de afiliação:
Argentina
País de publicação:
Reino Unido