On-line preconcentration and speciation analysis of inorganic vanadium in urine using l-methionine immobilised on controlled pore glass.
Talanta
; 74(4): 593-8, 2008 Jan 15.
Article
em En
| MEDLINE
| ID: mdl-18371680
A study was undertaken to ascertain the analytical capabilities of combined ICP-OES with ultrasonic nebulization to perform on-line preconcentration and speciation of inorganic V species in urine samples using a micro-column packed with l-methionine immobilized on controlled pore glass (CPG) as solid phase extractant. At pH 5.0, l-methionine is selective only towards V(V) while, total vanadium was quantitatively adsorbed by the solid phase at pH 9.0 [as V(V)] due to V(IV) oxidation in alkali media. Vanadium species retained by l-methionine were quantitatively eluted from the column with 10% HCl. Effects of acidity, sample flow rate, concentration of eluent and interfering ions on the recovery of the analytes have been systematically investigated. The detection limit (3sigma) of V is 0.008ngmL(-1) for USN-ICP-OES with an enhancement factor of 900 (10 for USN and 90 for l-methionine), and the relative standard deviation (R.S.D.) is better than 2%. The proposed method has been applied to the determination of inorganic V(V) and V(IV) in urine.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Vanádio
/
Vidro
/
Metionina
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Revista:
Talanta
Ano de publicação:
2008
Tipo de documento:
Article
País de afiliação:
Argentina
País de publicação:
Holanda