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Enhanced micropropagation response and biocontrol effect of Azospirillum brasilense Sp245 on Prunus cerasifera L. clone Mr.S 2/5 plants.
Russo, Anna; Vettori, Lorenzo; Felici, Cristiana; Fiaschi, Grazia; Morini, Stefano; Toffanin, Annita.
Afiliação
  • Russo A; Department of Biological and Environmental Sciences and Technologies, University of Lecce, 73100 Lecce, Italy. annalena.russo@unile.it <annalena.russo@unile.it>
J Biotechnol ; 134(3-4): 312-9, 2008 Apr 30.
Article em En | MEDLINE | ID: mdl-18358553
Inoculation with Azospirillum brasilense Sp245 exerts beneficial effects on micropropagated plants of Prunus cerasifera L. clone Mr.S 2/5, as seen in the results of a comparative analysis of inoculated and non-inoculated explants, during both the rooting and acclimatation phases. The presence of Azospirillum brasilense Sp245 increased root system, root hair biomass production and apical activity. Although the presence of the bacteria had a positive effect on rooting, the addition of indolebutyric acid (IBA) to Murashige and Skoog (MS) medium was seen as indispensable in order to promote the rooting of explants. Aside from the promotion of plant growth, A. brasilense Sp245 protects plants against pathogen attacks, such as Rhizoctonia spp., with a plant survival rate of nearly 100% vs. 0% as seen in the negative control. The biocontrol effect of A. brasilense Sp245 on the fungal rhizospheric community has been confirmed by denaturing gradient gel electrophoresis (DGGE) profiles of the rhizospheric microbial community. This study indicates that A. brasilense Sp245 could be employed as a tool in plant biotechnology.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Azospirillum brasilense / Controle Biológico de Vetores / Prunus País/Região como assunto: America do sul / Brasil Idioma: En Revista: J Biotechnol Assunto da revista: BIOTECNOLOGIA Ano de publicação: 2008 Tipo de documento: Article País de publicação: Holanda

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Azospirillum brasilense / Controle Biológico de Vetores / Prunus País/Região como assunto: America do sul / Brasil Idioma: En Revista: J Biotechnol Assunto da revista: BIOTECNOLOGIA Ano de publicação: 2008 Tipo de documento: Article País de publicação: Holanda