Simultaneous voltammetric detection of ascorbic acid, dopamine and uric acid using a pyrolytic graphite electrode modified into dopamine solution.
Anal Chim Acta
; 612(1): 89-98, 2008 Mar 31.
Article
em En
| MEDLINE
| ID: mdl-18331862
Pyrolytic graphite electrodes (PGE) were modified into dopamine solutions using phosphate buffer solutions, pH 10 and 6.5, as supporting electrolyte. The modification process involved a previous anodization of the working electrode at +1.5 V into 0.1 mol L(-1) NaOH followed by other anodization step, in the same experimental conditions, into dopamine (DA) solutions. pH of the supporting electrolyte performed an important role in the production of a superficial melanin polymeric film, which permitted the simultaneous detection of ascorbic acid (AA), (DA) and uric acid (UA), DeltaE(AA-DA)=222 mV; DeltaE(AA-UA)=360 mV and DeltaE(DA-UA)=138 mV, avoiding the superficial poisoning effects. The calculated detection limits were: 1.4x10(-6) mol L(-1) for uric acid, 1.3x10(-5)molL(-1) for ascorbic acid and 1.1x10(-7) mol L(-1) for dopamine, with sensitivities of (7.7+/-0.5), (0.061+/-0.001) and (9.5+/-0.05) A mol(-1) cm(-2), respectively, with no mutual interference. Uric acid was determined in urine, blood and serum human samples after dilution in phosphate buffer and no additional sample pre-treatment was necessary. The concentration of uric acid in urine was higher than the values found in blood and serum and the recovery tests (92-102%) indicated that no matrix effects were observed.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Ácido Ascórbico
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Ácido Úrico
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Dopamina
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Eletroquímica
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Eletrodos
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Grafite
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Revista:
Anal Chim Acta
Ano de publicação:
2008
Tipo de documento:
Article
País de afiliação:
Brasil
País de publicação:
Holanda