Your browser doesn't support javascript.
loading
Phenotypic and genotypic heterogeneity of Enterococcus species isolated from food in Southern Brazil.
Pelicioli Riboldi, Gustavo; Preusser de Mattos, Eduardo; Guedes Frazzon, Ana Paula; Alves d'Azevedo, Pedro; Frazzon, Jeverson.
Afiliação
  • Pelicioli Riboldi G; Programa de Pós-Graduação em Biologia Celular e Molecular, Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul.
J Basic Microbiol ; 48(1): 31-7, 2008 Feb.
Article em En | MEDLINE | ID: mdl-18247393
Enterococcus is an important group of lactic acid bacteria (LAB), which inhabits the gastrointestinal tract of humans and animals. These microorganisms can also be found in large groups of foods where they can play a beneficial role during food maturation processes or, conversely, can be used as a food contamination indicator. These microorganisms have an additional importance in various aspects of clinical microbiology. The aim of this study was to investigate and determine the phenotypic and genetic diversity in 55 enterococci isolated from different food sources. Phenotypic characteristics based upon substrate hydrolysis differences were used to identify different Enterococcus species. Analysis of the resultant data divided these species into eight Enterococcus phenotype groups. E. faecalis was the food isolate species with the greatest phenotypic variability. Fifty-five previously isolated Enterococcus strains were re-confirmed as belonging to this genus by PCR techniques. Randomly amplified polymorphic DNA (RAPD-PCR) was used to study the genetic variability using M13 primers and the resultant DNA fragments produced a database of different fingerprints. After statistical analyses of the RAPD-PCR profiles, 42 patterns were obtained and 6 different Enterococcus RAPD clusters (ERC) were identified. Genetic diversity was highest in ERC I, which grouped together approximately 40% of the E. faecium and E. faecalis isolates obtained from dairy products. Samples isolated from meat and vegetables offered the greatest genotype variability. Results of the present study suggest the presence of both phenotypic and genotypic variability within enterococci strains isolated from diverse sources of food common to Southern Brazil.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Variação Genética / Enterococcus / Microbiologia de Alimentos Tipo de estudo: Prognostic_studies Limite: Animals / Humans País/Região como assunto: America do sul / Brasil Idioma: En Revista: J Basic Microbiol Assunto da revista: MICROBIOLOGIA Ano de publicação: 2008 Tipo de documento: Article País de publicação: Alemanha

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Variação Genética / Enterococcus / Microbiologia de Alimentos Tipo de estudo: Prognostic_studies Limite: Animals / Humans País/Região como assunto: America do sul / Brasil Idioma: En Revista: J Basic Microbiol Assunto da revista: MICROBIOLOGIA Ano de publicação: 2008 Tipo de documento: Article País de publicação: Alemanha