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Expressed sequence tag analysis and development of gene associated markers in a near-isogenic plant system of Eragrostis curvula.
Cervigni, Gerardo D L; Paniego, Norma; Díaz, Marina; Selva, Juan P; Zappacosta, Diego; Zanazzi, Darío; Landerreche, Iñaki; Martelotto, Luciano; Felitti, Silvina; Pessino, Silvina; Spangenberg, Germán; Echenique, Viviana.
Afiliação
  • Cervigni GD; Centro de Recursos Naturales Renovables de la Zona Semiárida-CONICET, Camino de La Carrindanga Km 7.0, Bahia Blanca, Argentina.
Plant Mol Biol ; 67(1-2): 1-10, 2008 May.
Article em En | MEDLINE | ID: mdl-18196464
Eragrostis curvula (Schrad.) Nees is a forage grass native to the semiarid regions of Southern Africa, which reproduces mainly by pseudogamous diplosporous apomixis. A collection of ESTs was generated from four cDNA libraries, three of them obtained from panicles of near-isogenic lines with different ploidy levels and reproductive modes, and one obtained from 12 days-old plant leaves. A total of 12,295 high-quality ESTs were clustered and assembled, rendering 8,864 unigenes, including 1,490 contigs and 7,394 singletons, with a genome coverage of 22%. A total of 7,029 (79.11%) unigenes were functionally categorized by BLASTX analysis against sequences deposited in public databases, but only 37.80% could be classified according to Gene Ontology. Sequence comparison against the cereals genes indexes (GI) revealed 50% significant hits. A total of 254 EST-SSRs were detected from 219 singletons and 35 from contigs. Di- and tri- motifs were similarly represented with percentages of 38.95 and 40.16%, respectively. In addition, 190 SNPs and Indels were detected in 18 contigs generated from 3 to 4 libraries. The ESTs and the molecular markers obtained in this study will provide valuable resources for a wide range of applications including gene identification, genetic mapping, cultivar identification, analysis of genetic diversity, phenotype mapping and marker assisted selection.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Etiquetas de Sequências Expressas / Eragrostis Tipo de estudo: Risk_factors_studies Idioma: En Revista: Plant Mol Biol Assunto da revista: BIOLOGIA MOLECULAR / BOTANICA Ano de publicação: 2008 Tipo de documento: Article País de afiliação: Argentina País de publicação: Holanda

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Etiquetas de Sequências Expressas / Eragrostis Tipo de estudo: Risk_factors_studies Idioma: En Revista: Plant Mol Biol Assunto da revista: BIOLOGIA MOLECULAR / BOTANICA Ano de publicação: 2008 Tipo de documento: Article País de afiliação: Argentina País de publicação: Holanda