Milk secretory IgA related to Shigella virulence antigens.
Adv Exp Med Biol
; 310: 369-73, 1991.
Article
em En
| MEDLINE
| ID: mdl-1809012
PIP: 20 Mexico City and 23 Houston, Texas colostrum samples, and 21 Mexican and 25 Houston mature milk samples were analyzed by ELISA and Western blot, respectively, for antibodies against the virulence plasmid of Shigella flexneri serotype 5 strain M9OT. The method involved comparing water extracts of milk in ELISA and Western blot determinations of antigens against shigella flexneri strain M9OT which is fully virulent, to those against M9OT A2 which lacks the virulence plasmid. While there are at least 37 know distinct lipopolysaccharide antigens on different strains of the 4 species of Shigella, all contain the same plasmid conferring virulence, the ability of the bacteria to invade mammalian cells. This provided a universal test for antigens to Shigella. Western blots showed antibodies in all 21 Mexican women and in 40% of 25 Houston women. Plasmid antibodies were detected by ELISA in all 20 Mexican colostrum samples and in 52% of 23 Houston colostrum samples. After 8 days of lactation, 93% of the Mexican and 46% of the Houston milk samples were positive. The actual protective factor in human milk against Shigella bacteria is unknown: these findings suggest a mechanism for protection against all serotypes of shigella. The high prevalence of antibodies against Shigella found in Houston women was attributed to infection in the distant past.^ieng
Palavras-chave
Americas; Antibodies; Antibody Formation; Bacterial And Fungal Diseases; Biology; Developed Countries; Developing Countries; Diarrhea; Diseases; Human Milk; Immunity; Immunity, Active; Immunity, Natural; Immunologic Factors; Infections; Lactation; Latin America; Maternal Physiology; Mexico; North America; Northern America; Physiology; Research Report; Texas; United States
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Shigella
/
Imunoglobulina A Secretora
/
Leite Humano
/
Anticorpos Antibacterianos
/
Antígenos de Bactérias
Tipo de estudo:
Risk_factors_studies
Limite:
Female
/
Humans
País/Região como assunto:
Mexico
Idioma:
En
Revista:
Adv Exp Med Biol
Ano de publicação:
1991
Tipo de documento:
Article
País de publicação:
Estados Unidos